林耀輝

林耀輝
Lin, Yaw-Huei
退休通信研究員

美國加州大學洛杉磯分校博士 (1974)

TEL: 

+886-2-2787-1172 (Office)
+886-2-2787-1059 (Lab)

本研究室對甘薯的 trypsin inhibitors (以下簡稱TI) 自1980年起就陸續展開一系列基礎和應用的研究工作,奠定了甘薯TIs獨特的生理、生化功能 (即除anti-trypsin之外,兼具storage、stress-responsive及其他酵素活性之功能) 的實驗基礎。1997年更進一步找到SPTIs共價接著polyamines乃世界首次發現polyamines共價接到生理、生化功能已知之大分子者。近五年我們除了繼續努力於新發現外,並不斷找到直接或間接支持本研究室的發現之文獻。

從基礎研究到實際應用以回饋社會也有突破性的進展。曾總協調之NSC整合型計畫已把 SPTI gene 轉殖花椰菜成功,此轉殖花椰菜能抗斜紋夜盜蛾和小菜蛾。近五年來以水稻芽為材料在TI、peroxidase 和 plant growth regulators 也有重要發現已發表在國外著名期刊。

大陸推廣面積最廣的品種及潛力看好品種當中Nancy Hall及其後代高居第一,且遠超過其他品種系統。日本重要甘薯品種之一 Kokei 14 也是Nancy Hall 之後代(female Nancy Hall x male Siam)。因為大陸、台灣、日本佔全世界甘薯種植面積之絕大多數,所以這些地區的育種資料給申請人之"甘薯TIs的生理功能多樣性"假說提供最有力的驗證。臺灣大學農化系蘇仲卿教授及植物系林秋榮教授所指導的學生論文分別證實日本人發表之甘薯sporamin具TI活性;1996 年 Scott & Symes (Biochem. Mol. Biol. Inter. 38:333-344) 也證實本研究室之報告。

本研究室在甘薯找到之 protease 或 proteinase,如tripeptidyl peptidases,可切 L-methionine-4-nitroanilide, cysteinyl-specific peptidase (美國化學會 J. of Agr. and Food Chem.) 都是從未在植物體發現過的。

近五年來本研究室在植物蛋白酶及其大分子之抑制劑的實驗技術上也有重要突破 (統合proteases之spectrophotometric determination及活性染法 (Electrophoresis, 1999, 20:486-490) 及創新PAGE gels上三種酵素或inhibitors之活性染法 (Electrophoresis, 1998, 19: 212-214; ibid, 1998, 19: 692-694; ibid, 1999, 20:486-490; ibid, 2001, 22: 2653-2655)。其中 Electrophoresis, 1998, 19: 212-214 一文幫忙解除了植物生化工作者無法在電泳膠片上獲得可靠的葉 (以甘藷葉為代表) 抽取液TI活性染之困境。

從1946年M. Kunitz發表大豆的TI之後迄今,植物體TI對植物本身生理、生化之調控詳情一直未明;直到發現SPTIs共價接著polyamines才有了具體的線索。此為一系列工作之開始。接著申請人又發現 SPTIs兼具Dehydroascorbate reductase and monodehydroascorbate reductase之活性;以及"Dioscorin, the major tuber storage protein of Yam (Dioscorea batatas Decne) with carbonic anhydrase and trypsin inhibitor activities" 正說明植物塊根或塊莖的貯存性蛋白兼具多種生理功能之廣泛性。

近五年本研究室之研究成果可簡述如下:
1. 全世界首先發現植物 (綠豆) 之mitochondria含一複合體其兼具trypsin inhibitor 及 dehydroascorbate reductase 之活性 (J. Exp. Bot, 2000, 51: 713-719)。

2. 首先發現老化甘藷葉有isocitrate lyase 之gene transcription (J. Plant Physiology, 2000, 157: 669-676).

3. 首先發現甘藷TI及山藥之dioscorins都有antioxidant之活性 (Plant Science, 1999, 149: 151-156; J. Agri. Food Chem, 2001, 49: 2978-2981; ibid, 2001, 49: 4956-4960)。

4. 發現Ca2+-dependent and Ca2+-independent excretion modes of salicylic acid in tobacco cell suspension culture (J. Exp. Bot., 2001, 52: 1219-1226).

5. 發現甘藷內在aspartic type protease 可水解甘藷TI (Bot. Bull. Acad. Sin.,2002, 43: 271-276).

6. 發現甘藷TI可抑制甘藷塊根之內在serine type protease (Plant Science, 2002, 163: 733-739).

7. 發現可能啟動代謝甘藷TI之系統 (Plant Science, 2004, 166/2: 515-523).

8. Clone 且定性一甘藷之 asparaginyl endopeptidase c-DNA (J. Exp. Bot., 2004, 55/398: 825-835).

9. 發現兩種甘藷品系之TI 及其pepsin 水解物均具抗氧化能力 (Plant Science, 2005, 168: 449-456).

10. Plant Physiology 之 plant gene register 共兩件。

11. GenBank 之DNA sequences 共44件。

All publication list

 

Selected publication list

 

  • Huang D.J., H.J. Chen, W.C. Hou, T.E. Chen and Lin, Y.H.*. 2004. In vitro reduction of trypsin inhibitor by purified NADPH/thioredoxin system from sprouts of sweet potato (Ipomoea batatas (L) Lam.) storage roots. Plant Science 166/2: 435-441.
     
  • Huang D.J., H.J. Chen, W.C. Hou and Lin, Y.H.*. 2004. Isolation and characterization of thioredoxin h cDNA from sweet potato (Ipomoea batatas [L.] Lam "Tainong 57") storage roots. Plant Science 166/2: 515-523.
     
  • Chen, H.J., W.C. Hou, J.S. Liu, C.Y. Yang, D.J. Huang, and Lin, Y.H.*. 2004. Molecular cloning and characterization of a cDNA encoding asparaginyl endopeptidase from sweet potato (Ipomoea batatas (L.) Lam.) senescent leaves. Journal of Expermental Botany 55 (#398): 825-835.
     
  • Chen T.E., D.J. Huang, and Lin, Y.H.*. 2004. Isolation and characterization of a serine protease from the storage roots of sweet potato (Ipomoea batatas [L.] Lam) Plant Science 166: 1019-1026.
     
  • Huang D.J., C.D. Lin, H.J. Chen, and Lin, Y.H.*. 2004. Antioxidant and antiproliferative activities of sweet potato (Ipomoea batatas [L.] Lam "Tainong 57") constituents. Bot. Bull. Acad. Sin. 45: 179-186.
     
  • Huang D.J., H.J. Chen, W.C Hou, C.D. Lin, and Lin, Y.H.*. 2004. Active Recombinant Thioredoxin h Protein with Antioxidant Activities from sweet potato (Ipomoea batatas [L.] Lam "Tainong 57") storage roots. J. Agri. Food Chem. 52: 4720-4724
     
  • Hou, W.C., H.J. Chen, C.H. Han, C.Y. Lin, and Lin, Y.H.*. 2004. Glutathione peroxidase-like activity of 33 kDa trypsin inhibitor from roots of sweet potato (Ipomoea batatas (L.) Lam "Tainong 57"). Plant Science 166: 1541-1546.
     
  • Huang D.J., H.J. Chen, C.D. Lin, and Lin, Y.H.*. 2004. Antioxidant and antiproliferative activities of water spinach (Ipomoea aquatica Forsk) constituents. Bot. Bull. Acad. Sin. 46: 285-290
     
  • Hou, W.C., W.C. Wu, C.Y. Yang, H.J. Chen, S.Y. Liu, and Lin, Y.H.*. 2004. Antioxidant activities of methanolic and hot-water extracts from leaves of three cultivars of Mai-Men-Dong (Liriope spicata L.). Bot. Bull. Acad. Sin. 46: 285-290
     
  • Hou, W.C., C.H. Han, H.J. Chen, C.L. Wen, and Lin, Y.H.*. 2005. Storage proteins of two cultivars of sweet potato (Ipomoea batatas (L.) Lam) and their protease hydrolysates exhibited antioxidant activity in vitro. Plant Science 168: 449-456.
     
  • Huang D.J., H.J. Chen, W.C. Hou, T.E. Chen, W.Y. Hsu, and Lin, Y.H.*. 2005. Expression and function of a cysteine proteinase cDNA from sweet potato (Ipomoea batatas [L.] Lam "Tainong 57") storage roots. Plant Science 169: 423-431.
     
  • Huang D.J., H.J. Chen, and Lin, Y.H.*. 2005. Isolation and expression of protein disulfide isomerase cDNA from sweet potato (Ipomoea batatas) storage roots. Plant Science 169: 776-784.
     
  • Huang D.J., H.J. Chen, W.C. Hou, C.D. Lin, W.Y. Hsu, and Lin, Y.H.*. 2005. Sweet potato (Ipomoea batatas [L.] Lam "Tainong 57") storage root mucilage with antioxidant activities in vitro. Food Chemistry (in press).
     
  • Chen, H.J., D.J. Huang, W.C. Hou, J.S. Liu, and Lin, Y.H.*. 2005. Molecular cloning and characterization of a granulin-containing cysteine protease SPCP3 from sweet potato (Ipomoea batatas) senescent leaves. Journal of Plant Physiology (in press)
     
  • Huang, G.J., Huang, S.S., Chen, H.J., Chang, Y.S., Chag, S.J., Chang, H.Y., Hsieh, P.C., Chang, M.J., Lin, Y.C.,Lin, Y.H.*. 2009. Cloning and expression of aspartic proteinase cDNA from sweet potato storage roots. BOTANICAL STUDIES. 50/2: 149-158.
     
  • Chen, H.J., Huang, G.J., Chen, W.S., Su, C.T., Hou, W.C., Lin, Y.H. 2009. Molecular cloning and expression of a sweet potato cysteine protease SPCP1 from senescent leaves. BOTANICAL STUDIES. 50/2: 159-170.