張唯勤

張唯勤
Chang, Wei-Chin
通信研究員

美國加州大學河邊分校博士 (1970)

試管內形態發生

歷年來所從事的工作主要是由在試管內培養的組織和細胞中尋求特異的形態發生現象,以研討此等形態發生的控制因子,並用以開發一些實用的途徑。諸如人蔘 (Panax ginseng) 組織培養中之體胚會直接開花、結實、果實增殖成多量果狀物,以及由果實群中形成花器等。也進一步研討控制果實增殖與開花的因子。

三種竹類之組織培養中也有經由體胚開花之現象,並出現『花長花』之形態發生變化。此等系統正用來研究竹類開花不易的現象,以及如何縮短幼年期。經由體胚再生植株之體系也可用於殖入竹類鑲嵌紋病毒鞘基因,企圖建立抗病毒之竹株研究。

同時還進行多種蘭科植物之細胞培養,研究方向著重在開發細胞再生植株體系,以供從事轉殖蘭株之用。細胞再生之體系也提供大量繁殖蘭科植物的新方法。蘭科植物細胞培養中也有多量花器形成,因此目前我們已有五種植物(人蔘、素心蘭、三種竹類)之試管開花體系,都為研討此等植物生殖相的好材料。

 

 

Selected publication list

 

  • Wang, H.C., Chen, J.T. and Chang, W.C. 2010. Morphogenetic routes of long-term embryogenic callus culture of Areca catechu.” Biologia Plantarum 54: 1-5.
  • Lan T.H., Hong P.I., Huang, C.C., Chang W.C., and Lin C.S. 2009 Development and preliminary molecular characterization of a system for high-frequency direct somatic embryogenesis from leaf tissues of Drimiopsis kirkii Baker (Giant Squill). In Vitro Cellular and Developmental Biology - Plant 45:44-47
  • Gow, W.P.; Chen, J.T. and Chang, W. C. 2009. Effects of genotype, light regime, explant position and orientation on direct embryo formation from leaf explants of Phalaenopsis orchids. Acta Physiol plantarum 31: 363-369.
  • Hong, P.I., Chen, J.T. and Chang, W. C. 2008. Effects of salicylic and acetylsalicylic acid on direct somatic embryogenesis in Oncidium. J. Plant Biochem. & Biotech. 17: 149-153.
  • Hong, P.I., Chen, J.T. and Chang, W. C. 2008. Plant regeneration via protocorm-like body formation and shoot multiplication from seed-derived callus of a maudiae type slipper orchid. Acta Physiol plantarum (DOI 10.1007/s11738-008-0158-2)
     
  • Gow, W.P., Chen, J.T.* and Chang, W. C. 2008. Influence of growth regulators on direct embryo formation from leaf explants of Phalaenopsis orchids. Acta Physiol Plantarum 30: 507-512.
  • Hong, P.I., Chen, J.T. and Chang, W. C. 2008. Promotion of direct somatic embryogenesis of Oncidium by adjusting carbon sources. Biologia Plantarum. 52: 597-600.
     
  • Chung, H.H., Chen, J. T andChang, W. C. and 2007. Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. Biologia Plantarum 51: 346-350.
  • Chung, H.H., Chen, J.T. and Chang, W. C. and 2007. Zygotic and somatic embryogenesis in Phalaenopsis. Orchid Science and Biotechnology 1: 40-43.
  • Lin C.S., Kalpana, K., Lin, N.S. and Chang, W. C. 2007. Improving multiple shoot proliferation In BaMV-free Bambusa oldhamii Munro propagation by liquid culture. HortSci. 42:1243-1246.
  • Chang, W. C. 2007. "in vitro morphogenesis and micro-propagation of orchids" In: W.H. Chen and H.H. Chen (eds.) Orchid Biotechnology. World Scientific, Singapore. (In press)
  • Chang, W. C. 2007. In vitro morphogenesis and micropropagation of orchids. In.Proc.27th International Horticultural Congress. Seoul. Korea. Aug.13-18.2006. (In press)
     
  • Lin C.S., C.J. Liang, H.W. Hsaio, M.J. Lin, Chang, W. C. 2007. Flowering of the green and albino regenerates obtained via somatic embryogenesis in inflorescence segments of Dendrocalamus latiflorus. New Forests 34: 177-186.
     
  • Liu N.T., F.H. Wu., H.S. Tsay, Chang, W. C. C.S. Lin. 2007. Chloroplast genome aberration in micropropagation-derived albino mutants, ab1 and ab2. Plant Cell Tissue and Organ Culture 88: 147-156.
     
  • Lin, C. S., M. C. Tseng, P. I. Hong, Chang, W. C. 2006. Albino inflorescence proliferation of Dendrocalamus latiflorus. In Vitro Cell. Develop. Plant. 42: 336-340.
     
  • Lin, C. S., Y. H. Lai, C. W. Sun, N. T. Liu, H. S. Tsay, Chang, W. C. J. J. W. Chen. 2006. Identification of ESTs differentially expressed in green and albino mutant bamboo (Bambusa edulis) by suppressive subtractive hybridization (SSH) and microarray analysis. Plant Cell Tiss. Org. Cult. 86: 169-175.
     
  • Lin, C. S., H. L. Lin, H. W. Hsiao, W. N. Jane, C. C. Lin, F. Y. Jheng, Chang, W. C. 2006. Changes in the morphology and cation content of a Bambusa edulis xylem mutant, vse, derived from somaclonal variation. J. Amer. Soc. Hort. Sci. 131: 445-451.
     
  • Wang, H. C., J. T. Chen and Chang, W. C. 2006. Somatic embryogenesis and plant regeneration from leaf, root and stem- derived callus of Areca catechu. Biol. Plant. 50: 279-282.
  • Chen, J. T. and Chang, W. C. 2006. Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis. Biol. Plant. 50: 169-173.
     
  • Su, Y. J., Chen, J. T. and Chang, W. C. 2006.Efficient and repetitive production of leaf-derived somatic embryos of Oncidium. Biol. Plant. 50: 107-110.
     
  • Chung, H.H., Chen, J.T. and Chang, W. C. 2005. Cytokinins induce direct somatic embryogenesis of Dendrobium Chiengmai Pink and subsequent plant regeneration. In Vitro/Plant. 41: 765-769.
     
  • Kuo, H. L., Chen, J. T., and Chang, W. C. 2005. Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis 'Little Steve'. In Vitro-Plant. 41: 453-456.
     
  • Chang, W. C.and Drew, R. (eds.) 2005. Proceedings of the 2nd International Symposium on Biotechnology of Tropical and Subtropical Species. Acta Hort. 692.
     
  • Chen, J. T., Su, Y. J., Wu, I. F., Chueh, C. M., Kuo, H. J. and Chang, W. C. 2005. Somatic embryogenesis in Oncidium. In: Chang, W. C. , and Drew, R. (eds.) Proc. of the 2nd International Symposium on Biotechnology of Tropical and Subtropical Species. Acta Hort. 692: 75-80.
     
  • Chang, W. C. Chen J. T., Chang, C., Chen Y. C., Lin, Y. H., Su. Y. J., Chen, T. Y., M. C. Tseng, Kuo. H. L., Wu. I. F. and Chueh, C. M. 2005. In vitro morphogenesis of five orchids. In: Chang, W. C. , and Drew, R. (eds.) Proc. of the 2nd International Symposium on Biotechnology of Tropical and Subtropical Species. Acta Hort. 692: 115-118.
  • Lin, C. S., Cheng, M. J., Hsiao, H. W., Hong, P. I., Jheng, F. Y., Lin, C. C., and Chang, W. C. 2005. Stamen-less inflorescence proliferation of Bambusa edulis. Scientia Horticulturae 107: 76-80.
     
  • Lin, C. S., Chen, T. C., Hsiao, H. W., and Chang, W. C. 2005. Effect of growth regulator on direct flowering of dormant ginseng buds in vitro. Plant Cell Tiss. & Org. Cult. 83: 241-244.
     
  • Lin, C. S., Lin, C. C., andChang, W. C. 2005. Shoot regeneration, re-flowering and post flowering survival in bamboo inflorescence culture. Plant Cell Tiss. & Org. Cult. 82: 243-249.
     
  • Chang, C., Chen, Y. C., Hsu, Y. H., Wu, J. T. , Hu, C. C., Chang, W. C. and Lin, N. S. 2005. Transgenic resistance to Cymbidium Mosaic Virus in Dendrobium with the capsid protein gene. Transgenic Research 14: 41-46.
  • Chen, J. T., and Chang, W. C. 2004. TIBA affects the induction of direct somatic embryogenesis of Oncidium leaf explants. Plant Cell Tiss. & Org. Cult. 79: 315-320.
  • Lin, C. S., Vidmar, J., and Chang, W. C. 2004. Effects of growth regulators on inflorescence proliferation of Bambusa edulis. Plant Growth Regul. 43: 221-225.
  • Chen, J. T., and Chang, W. C. 2004. Induction of repetitive embryogenesis from seed-derived protocorms of Phalaenopsis amabilis var. formosa Shimadzu. In Vitro/Plant. 40: 290-293.
     
  • Lin, C. S., Lin, C. C., and Chang, W. C. 2004. Effects of auxins and cytokinins on embryo formation from callus of Oncidium 'Gower Ramsey'. Plant Cell Tiss. & Org. Cult. 77: 107-109.
     
  • Chen, T. Y., Chen, J. T., and Chang, W. C. 2004. Plant regeneration through shoot bud formation from leaf of Paphiopedilum orchids. Plant Cell Tiss. & Org. Cult. 76: 11-15.
     
  • Wang, H. C., Chen, J. T., Wu, S. P., Lin, M. C., and Chang, W. C. 2003b. Plant regeneration through shoot bud formation from shoot tip-derived callus of Areca catechu L. (Arecaceae). Plant Cell Tiss. & Org. Cult. 75: 95-98.
     
  • Wang, H. C., Chen, J. T., Wu, S. P., Lin, M. C., and Chang, W. C. 2003a. Plant regeneration through somatic embryogenesis from zygotic embryo-derived callus of Areca catechu L. (Arecaceae). In Vitro/Plant. 39: 34-36.
     
  • Lin, C. S., Chen, C. T., Lin, C. C., and Chang, W. C. 2003b. A method for inflorescence proliferation. Plant Cell Rep. 21: 838-843.
     
  • Lin, C. S., Lin, C. C., and Chang, W. C. 2003a. In vitro flowering of Bambusa edulis and subsequent plantlet survival. Plant Cell Tiss. & Org. Cult. 72: 71-78.
     
  • Chang, C., and Chang, W. C. 2003b. 1-aminocyclopropane-1-carboxylic acid enhances direct somatic embryogenesis for Oncidium leaf cultures. Biol. Plant. 46: 455-458.
  • Chang, C., and Chang, W. C. 2003a. Effects of GA3, ancymidol, cycocel and paclobutrazol on direct somatic embryogenesis of Oncidium in vitro. Plant Cell Tiss. & Org. Cult. 72: 105-108.
  • Chang, C., and Chang, W. C.2003. Cytokinins promotion of flowering in Cymbidium ensifolium var. misericors in vitro. Plant Growth Regul. 39: 217-221.
     
  • Chen, L. R., Chen, J. T., and Chang, W. C. 2002. Efficient production of protocorm-like bodies and plant regeneration from flower stalk explants of the sympodial orchid Epidendrum radicans. In vitro/Plant 38: 441-445.
     
  • Chen, T. Y., Chen, J. T., and Chang, W. C. 2002. Multiple shoot formation and plant regeneration from stem nodal explants of Paphiopedilum orchids. In vitro/Plant 38: 595-597.
     
  • Chen, J. T, and Chang, W. C. 2002. Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium 'Grower Ramsey'. Plant Cell Tiss. & Org. Cult. 69: 41-44.
     
  • Chen, J. T., and Chang, W. C. 2001. Effects of auxins and cytokinins on direct somatic embryogebesis on leaf explants of Oncidium 'Growth Ramsey'. Plant Growth Regul. 34: 229-232.
  • Chang, C., and Chang, W. C. 2000. Micropropagation of Cymbidium ensifolium var. misericors through callus-derived rhizomes. In Vitro/Plant 36: 517-520.
     
  • Chen, J. T., and Chang, W. C. 2000b. Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae). Plant Sci. 160: 87-93.
     
  • Chen, J. T., and Chang, W. C. 2000a. Plant regeneration via embryo and shoot bud formation from flower stalk explants of Oncidium "Sweet Sugar". Plant Cell Tiss. & Org. Cult. 62: 95-100.
     
  • Chen, Y. C., Chang, C., and Chang, W. C. 2000. A reliable protocol for plant regeneration from callus culture of Paphiopedium . In Vitro/Plant 36: 420-423.
     
  • Lin, Y. H., Chang, C., and Chang, W. C.2000. Plant regeneration from callus culture of a Paphiopedilum hybrid. Plant Cell Tiss. & Org. Cult. 62: 21-25.
     
  • Chang, C. and Chang, W. C. 2000. Effects of thidiazuron on bud development of Cymbidium sinense willd in in vitro. Plant Growth Regul. 30: 171-175.
     
  • Chen, J. T., Chang, C., and Chang, W. C. 1999. Direct somatic embryogebesis on leaf explants of Oncidium 'Grower Ramsey' and subsequent plant regeneration. Plant Cell Rep. 19: 145-149.
     
  • Lin, C., and Chang, W. C. 1998. Micropropagation of Bumbusa edulis through nodal explants of field-grown culms and flowering of regenerated plantlets. Plant Cell Rep. 17: 617-620.
     
  • Chang, C. and and Chang, W. C. 1998. Plant regneration from callus culture of Cymbidium ensifolium var misericors. Plant Cell Rep. 17: 251-255.
     
  • Chang, W. C. and Ho, C. W. 1997. Micropropagation of Bamboos. In: Biotechnology in Agriculture and Forestry. Y.P.S. Bajaj. vol 39, Hi-tech and Micropagation, p 201-219. Springer Verlag. Berlin.
     
  • Chang, W. C. and Lan, T. H. 1995. Somatic embryogenesis and plant regeneration from roots of bamboo (Bambusa beecheyana munro var beecheyana). J. Plant Physiol. 145: 535-538.
  • Chang, W. C. 1994. Somatic embryogenesis of Bambusa oldhammii, B. beecheyana and Sinocalamus latiflora. In: Somatic embryogenesis in Woody Plants. S. Jain, P. Gupta and R. Newton, eds, Kluwer Academic Publishers, Netherlands. pp 53-66.
     
  • Chang, W. C. 1991. Dysosma pleiantha (Hence) Woodson: somatic embryogenesis and plant regeneration. In: Biotechnology in Agriculture and Forestry, Vol 15: Medicinal and Aromatic Plants III, (Y. B. S. Bajaj ed.). p 237-245, Springer-Verlag, Berlin.
  • Chang, W. C. 1991. Bamboos In: Biotechnology in Agriculture and Forestry, Vol 16: Tree III (Y. B. S. Bajaj ed.). p 221-27, Springer-Verlag, Berlin.
     
  • Chuang, M. L. and Chang, W. C. 1987. Somatic embryogenesis and plant regeneration in callus culture derived from immature seeds and mature zygotic embryos of Dysosma pleiantha (Hance) Woodson. Plant Cell Rep. 6: 484-485.
     
  • Jong, T. M. and Chang, W. C.1987. Somatic embryogeneis and plant regeneration from inflorescence callus of Zinania latifolia Turcz. J. Plant Physiol. 130: 67-71.
     
  • Yeh, M. L. and Chang, W. C. 1987. Plant regeneration via somatic embryogenesis in mature embryo-derived callus culture of Sinocalamus latiflora (Munro) McClure. Plant Sci. 50: 93-96.
     
  • Chung, M. J. and Chang, W. C. 1986. Embryoid formation and plant regeneration in callus cultures derived from vegetative tissues of Dysosma pleiantha (Hance) Woodson. J. Plant Physiol. 128: 279-283.
     
  • Yeh, M. L. and Chang, W. C. 1986. Somatic embryogenesis and subsequnt plant regeneration from inflorescence of Bambusa beecheyana Munro var. beecheyana. Plant Cell Rep. 5: 409-411.
     
  • Yeh, M. L. and Chang, W. C. 1986. Plant regeneration though somatic embryogeneis in callus culture of green bamboo (Bambusa oldhamii Munro). Theor. Appl. Genet. 72: 161-163.
     
  • Chang, W. C. and Hsing, Y. E. 1980. Plant regeneration through somatic embryogenesis in root-derived callus of ginseng (Panax ginseng C. A. Meyer). Theor. Appl. Genet. 57: 133-135.
     
  • Chang, W. C. and Hsing, Y. E. 1980. In vitro flowering of embryoids derived from mature root callus of ginseng (Panax ginseng). Nature 284: 341-342.
     
  • Chang, W. C. and Chiu, P. L. 1978. Regeneration of Lemna gibba G3 through callus culture. Z. Pflanzenphysiol. 81: 91-94.
     
  • Chang, W. C. and Hsing, Y. I. 1978. DPX-3778 promotes the proliferation of tobacco callus in vitro. Planta (Berlin) 140: 293-294.