王中茹

王中茹
Wang, Chung-Ju
助研究員

2003 Ph.D. Dept. of Botany, Natl. Taiwan Univ.
2003-2004 PDF, IPMB, Academia Sinica.
2004-2010 PDF, Dept of Mol Cell Biology, UC-Berkeley, USA.
2011-present Ass. Res. Fellow, IPMB, Academia Sinica

TEL: 

+886-2-2787-1120 (Office)
+886-2-2787-1119 (Lab)

減數分裂是將染色體數目成套減半的特化細胞分裂,在真核生物有性生殖中扮演決定性的角色。本實驗室致力於了解在植物中,減數分裂起始及終止的調控,以及在減數分裂初期發生的同源染色體配對與重組的分子機制。

減數分裂如何啟始?

在植株生長的後期,一小群體細胞在花藥及胚珠裡分化成減數分裂細胞。細胞如何將有絲分裂轉換成減數分裂是重要的生物學課題。我們藉由標定花藥裡減數分裂細胞的DNA複製,發現減數分裂開始前,所有細胞經過一段特化的細胞週期暫停,以使減數分裂S週期同步起始。我們並發現MAC1和AM1分別對於細胞週期同步化和進入減數分裂S期非常重要。我們目前透過對其功能的分析,以及系統性地了解轉錄組及蛋白質體在其中的變化,以了解關鍵的基因和機制。

減數分裂如何結束?

在被子植物中,減數分裂結束後,單倍體胞子必須重新進行有絲分裂以產生配子體,也就是成熟花粉粒或是胚囊。細胞如何終止減數分裂,轉換成有絲分裂也少有研究。玉米po1突變株由George Beadle在1929年分離出來,其表型為減數分裂第二時期結束後,卻再次發生染色體分離。我們發現PO1為一個具TPR模組的蛋白質,利用定量定性的蛋白質體學分析,我們發現PO1可能透過調節末期促進複合體,影響蛋白質降解來控制減數分裂週期。

同源染色體如何完成配對及重組?

減數分裂第一時期中,同源染色體彼此配對並發生DNA重組。同時,聯會複合體在同源染色體間形成,緊密的連結同源染色體並調節染色體間的重組。染色體間的配對,聯會和基因重組皆為減數分裂時重要的過程,以產生單配體的配子細胞。染色體如何認得其同源染色體?又如何與聯會複合體之形成彼此協調?染色體重組的位置如何決定?都是非常重要又有趣的問題。我們藉著研究數個參與的蛋白質,來了解配對及聯會這個相當動態的過程。

All publication list

 

Selected publication list

 

  • Lambing C, Franklin FCH, Wang CJR* (2017). Understanding and manipulating meiotic recombination in plants. Plant Physiology,173 (1): DOI:10.1104/pp.16.01530.
  • Lee DH, Kao YH, Ku JC, Lin CY, Meeley R, Jan YS, Wang CJR* (2015). The axial element protein DESYNAPTIC2 mediates meiotic double-strand break formation and synaptonemal complex assembly in maize. Plant Cell, 27:2519-2529.    
  • Wang CJR*, Tseng CC (2014). Recent advances in understanding of meiosis initiation and the apomictic pathway in plants. Frontiers in Plant Science, 5, 497:1-6.
  • Wang CJR* (2013). Analyzing maize meiotic chromosomes with structured illumination microscopy. Editor(s): Pawlowski WP, Grelon M, Armstrong S, Methods in Molecular Biology, pp 67-78. New York: Springer (Invited book chapter)
  • Moon J, Skibbe D, Timofejeva L, Wang CJR, Kelliher T, Kremling K, Walbot V, Cande WZ* (2013). Regulation of cell divisions and differentiation by MALE STERILITY32 is required for anther development in maize. Plant Journal, 76:592-602.
  • Timofejeva L, Skibbe D, Lee S, Golubovskaya IN, Wang CJR, Harper L, Walbot V, Cande WZ* (2013). Cytological characterization and allelism testing of anther developmental mutants identified in a screen of maize male sterile lines. G3, 3:231-249.
  • Wang CJR, Nan G, Kelliher T, Timofejeva L, Vernoud V, Golubovskaya IN, Harper L, Egger R, Walbot V, Cande WZ* (2012). Maize Multiple archesporial cells 1 (Mac1), an ortholog of rice TDL1A, modulates cell proliferation and identity in early anther development. Development, 139:2594-2603.
  • Paredez AR, Assaf ZJ, Sept D, Timofejeva L, Dawson SC, Wang CJR, Cande WZ* (2011). An actin cytoskeleton with evolutionarily conserved functions in the absence of canonical actin-binding proteins. Proc Natl Acad Sci USA, 108:6151-6156.
  • Nan GL, Ronceret A, Wang CJR, Fernandes JF, Cande WZ, Walbot V* (2011). Global transcriptome analysis of two ameiotic1 alleles in maize anthers: defining steps in meiotic entry and progression through prophase I. BMC Plant Biology, 11:120.
  • Golubovskaya IN, Wang CJR, Timofejeva L, Cande WZ* (2011). Maize meiotic mutants with improper or nonhomologous synapsis due to problems in pairing or synaptonemal complex formation. Journal of experimental botany, 62:1533-1544. (The first two authors contributed equally to this work) (Journal cover). 

博士後研究 Postdoctoral Research Associate
Jeremy Catinot
李頂華 Ding-Hua Lee
鄭舒允 Shu-Yun Chen
張珮君 Pearl Chang 

研究助理 Research Assistant
古家齊 Jia-Chi Ku
高玉馨 Yu-Hsin Kao
王琪婷 Chi-Ting Wang

博士班研究生 Ph.D Student
曾靜枝 Ching-Chih Tseng
Mitylene Bailey