麥東倈

麥東倈
Matzke, Antonius
特聘研究員

Postdoctoral studies, plant molecular biology, Washington University, St. Louis, supervisor: Dr. Mary-Dell Chilton(1980-1982)

Ph.D. Biochemistry, Univ. of Vienna, Austria; Advisor: Dr. Ernst Küchler Dissertation title: Concerning the mechanism of ribosomal translocation(1977-1979)

M.S. Biochemistry, Univ. of Vienna, Austria; Advisor: Dr. Ernst Küchler
Thesis title: UV-induced cross-linking between the anticodon region of tRNA and mRNA in E. coli ribosomes(1976-1977)

B.S. Biochemistry Univ. of Vienna, Austria(1972-1976)

TEL: 

+886-2-2787-1136(Dr.Antonius)
+886-2-2787-1135(Dr.Marjori)
+886-2-2787-1137(Lab)

研究主題:植物的基因表現調控

本實驗室是在研究調控植物基因表現的因子。不同的基因表現造成的各類型細胞的特化,也造就了生長發育階段的差異及植物對逆境的反應等等。早期的研究是著重在表觀遺傳調控、轉錄基因靜默以及一個特殊的、經由核醣核酸(RNA) 所引導的去氧核醣核酸(DNA)的甲基化過程。近期的研究結果開創了新的研究方向,也就是大家所熟知的「訊息核醣核酸前體的選擇性剪切」(alternative splicing of premRNA)。這個領域包含了RNA轉錄時及轉錄後的調控。

研究策略是由遺傳篩查來找出調控缺失的突變體,再用基因體定序的方式定位突變體中的基因缺失,這些有缺失的基因即為潛在的調控因子,後續再用分生或生化的方式分析這些因子在整個機制中的功能。此遺傳篩查是建立在實驗室已發展出的一套系統----帶有綠色螢光蛋白(GFP) 報導基因的阿拉伯芥轉殖株 ( 圖1) ,此報導基因上游帶有一段會和GFP同時被轉錄的片段,若它必須被正確剪切以轉譯出正常的GFP。在誘導突變之後,我們篩選螢光強度有改變( 變強或變弱) 的突變體。目前利用此策略已篩出先前未知的因子,其中有些可能和植物對逆境的反應相關,這些因子可能會是農業生物技術發展的標的。之後的篩選應當能夠發現更多選擇性剪切或逆境適應的未知分子,讓擴充我們對這些生理現象的知識庫。

細胞間期的染色體組織也是研究主題之一,因為它影響基因表現的機制尚未明瞭。目前階段是觀察非整倍體(aneuploids) 中的基因表現及DNA甲基化的分佈情形,以及多餘的染色體所造成的生物物理現象的改變( 比如細胞核膜電位的改變) 。我們利用神經科學家研發出的電
位指示螢光蛋白(genetically-encoded fluorescentvoltage indicators, GEVIs) 來設計以螢光為訊號的植物電生理研究。因為GEVIs能夠被表現在細胞的內膜細統裡( 圖2) ,這使得以往深藏在細胞裡、難以用傳統電生理儀器測量的內膜系統電位得以被研究。

 


Figure 1. GFP phenotypes of mutant seedlings: left, wild-type T line; middle, GFPweak mutant (reduced expression); right, HyperGFP mutant (enhanced expression)


Figure 2. GEVI localized to inner nuclear membrane of Arabidopsis root cells

 

All publication list

 

Selected publication list

 

  • Kanno T, Lin WD, Fu JL, Wu MT, Yang HW, Lin SS, Matzke AJM*, and Matzke M* (2016) Identifi cation of coilin
    mutants in a screen for enhanced expression of an alternatively spliced GFP reporter gene in Arabidopsis thaliana.
    Genetics 203:1709 1720.

  • Matzke AJM, Matzke M* (2015) Expression and testing in plants of ArcLight, a genetically-encoded voltage indicator used
    in neuroscience research. BMC Plant Biol 15:245.

  • Sasaki T, Kanno T, Liang SC, Chen PY, Liao WW, Lin WD, Matzke AJM*, Matzke M*. (2015) An Rtf2 domain-containing
    protein influences pre-mRA splicing and is essential for embryonic development in Arabidopsis thaliana. Genetics 200:
    523-535.

  • Matzke M, Kanno T, Matzke AJM*. (2015) RNA-directed DNA methylation: the evolution of a complex epigenetic pathway
    in flowering plants. Annu Rev Plant Biol 66: 243-267.

  • Sasaki T, Lee TF, Liao WW, Naumann U, Liao JL, Eun C, Huang YY, Fu JL, Chen PY, Meyers BC, Matzke AJ*, Matzke M*.
    (2014) Distinct and concurrent pathways of Pol II and Pol IV-dependent siRNA biogenesis at a repetitive trans-silencer
    locus in Arabidopsis thaliana. Plant J 79: 127-138.

  • Huang YY, Lee CP, Fu JL, Chang BC, Matzke AJM*, Matzke M*. (2014) De novo transcriptome sequence assembly from
    coconut leaves and seeds with a focus on factors involved in RNA-directed DNA methylation. G3: Genes Genomes
    Genetics 4: 3147-2157.

  • Matzke MA*, Mosher RA. (2014) RNA-directed DNA methylation: an epigenetic pathway of increasing complexity. Nat Rev
    Genet 15: 394-408.

  • Matzke AJM*, Matzke M. (2013) Membrane ‘potential-omics’: towards voltage imaging at the cell population level in roots
    of living plants. Front Plant Sci 4: 311.

  • Lorkovic ZJ, Naumann U, Matzke AJM, Matzke M*. (2012) Involvement of a GHKL ATPase in RNA-directed DNA methylation
    in Arabidopsis thaliana. Curr Biol 22: 933-938.

  • Kanno T, Bucher E, Daxinger L, Huettel B, Kreil DP, Breinig F, Lind M, Schmitt MJ, Simon SA, Gurazada SG, Meyers
    BC, Lorkovic ZJ, Matzke AJM, Matzke M*. (2010) RNA-directed DNA methylation and plant development require an
    IWR1-type transcription factor. EMBO Reports 11: 65-71.

  • Matzke AJM, Watanabe K, van der Winden J, Naumann U, Matzke M*. (2010) High frequency, cell type-specific visualization
    of fluorescent-tagged genomic sites in interphase and mitotic cells of living Arabidopsis plants. Plant Methods 6: 2

 

Tatuso Kanno
菅野達夫

Research Scientist
研究助技師

   

Chia-liang Chang
張家亮

Research Assistant
資深研究助理

   

Phebe Hui Chiou
邱蕙

Research Assistant
研究助理

   

Christine Ya-Ching Ying
應雅青

Lab Manager
實驗室助理