王昭雯

王昭雯
Wang, Chao-Wen
副研究員

美國加州大學戴維斯分校 細胞學及發育生物學博士 (2003)

1995 B.S. Dept. Plant Pathology & Entomology, Natl. Taiwan Univ.

1997 M.S. Dept. Plant Pathology & Entomology, Natl. Taiwan Univ.

2003 Ph.D. Cell and Dev. Biol., UC-Davis, USA

2003-2004 PDF. Mol Cell Dev. Biol., UMich-Ann Arbor, USA

2004-2007 PDF. Mol Cell Biology, UC-Berkeley, USA

2007-2012 Asst. Res. Fellow, IPMB, Academia Sinica

2012-present Asso. Res. Fellow, IPMB, Academia Sinica

TEL: 

+886-2-2787-1173 (Office)
+886-2-2787-1060 (Lab)

分子細胞生物學

真核生物細胞利用各種的胞器將繁複的生命機制加以分類,而這些胞器通常都有它特定的形態和功能剖析胞器的形態和功能是分子細胞生物學的重點也是我們長期的研究興趣我們目前研究鎖定的是一個細胞內用來儲存中性脂的胞器它叫做油滴這個胞器被認為是從內質網的外膜形成的油滴的結構很簡單它是一層的磷脂質包裹著中性脂,而油滴中儲存的中性脂除了可以提供細胞中能量的來源,同時也是用於建構細胞內膜系統的原料,也因此油滴是細胞中專司油脂代謝的胞器。我們將研究的核心問題鎖定在細胞如何產生油滴油滴的結構如何維持細胞又如何來控制油滴的大小和數目。此外,油滴是一個非常具有活動力的胞器它會與很多不同的胞器之間進行互動我們也想知道這些互動模式對油滴的功能和油脂代謝有什麼關連性。

我們的研究策略是從最簡單的酵母菌細胞出發,並逐步利用多細胞生物模式系統來探究油滴可能參與的機制和生理功能。我們首先利用遺傳學的方法在酵母菌中找出影響油滴功能的各式突變株,利用生物影像等細胞學研究法觀察油滴的動態以推測油滴的各種可能作用模式,同時這個方法也幫助我們釐清一些特定分子被帶到油滴上的可能機制和調控,我們更進一步利用生物化學和分子生物學的研究法分析特定蛋白和油滴的關聯性並試圖綜合這些證據來全面瞭解油滴在生成和作用上的分子機制。由於油滴的變化跟人類很多油脂代謝疾病有強烈的關聯性,油滴中儲存的油脂同時也是生質能源的極佳材料,我們將從最基礎的研究層面去瞭解油滴,並期許這個基礎研究的成果可以運用於解釋上述不同層次的生命科學問題。

All publication list

 

Selected publication list

 

  • Hsu, T.-H., Chen, R-H., Cheng, Y.-H., and Wang, C.-W.* (2017) Lipid droplets are central organelles for meiosis II progression during yeast sporulation. Mol. Biol. Cell. pii: mbc.E16-06-0375.
  • Iwasa S., Sato, N., Wang, C.-W., Cheng, Y.-H., Irokawa, H., Hwang, G-W., Naganuma, N., and Kuge, S. (2016). The Phospholipid:Diacylglycerol Acyltransferase Lro1 Is Responsible for Hepatitis C Virus Core-Induced Lipid Droplet Formation in a Yeast Model System. PLoS ONE. 11(7):e0159324.
  • Yang, P.-L., Hsu, T.-H., Wang, C.-W.*. and Chen, R-H.* (2016) Lipid droplets maintain lipid homeostasis during anaphase for efficient cell separation in budding yeast. Mol. Biol. Cell. 2016 Aug 1;27(15):2368-80.
  • Wang, C.-W.* (2016). Lipid droplets, lipophagy, and beyond. Biochim Biophys Acta. 1861(8 Pt B):793-805.
  • Wang, C.-W.* (2015). Lipid droplet dynamics in budding yeast. Cell. Mol. Life Sci. 72(14):2677-95.
  • Peng, K.-C., Wang, C.-W., Wu, C.-H., Huang, C.-T., Liou, R.-F. (2015). Tomato SOBIR1/EVR homologs are involved in elicitin perception and plant defense against the oomycete pathogen Phytophthora parasitica. Mol. Plant Microbe Interact. 28(8):913-26.
  • Wang, C.-W.* (2014). Stationary phase lipophagy as a cellular mechanism to recycle sterols during quiescence. Autophagy. 10(11): 2075-6.
  • Wang, C.-W.*, Miao, Y.-H., and Chang, Y.-S. (2014). A sterol-enriched vacuolar microdomain mediates stationary phase lipophagy in budding yeast. J. Cell Biol.206(3):357-66.
  • Wang, C.-W.*, Miao, Y.-H., and Chang, Y.-S. (2014). Control of lipid droplet size in budding yeast requires the collaboration between Fld1 and Ldb16. J. Cell Sci. 127: 1214-1228.
  • Starr, T. L., Pagant, S., Wang, C.-W., and Schekman, R. (2012). Sorting signals that mediate traffic of chitin synthase III between the TGN/endosomes and to the plasma membrane in yeast. PLoS ONE. 7(10): e46386.
  • Wang, C.-W.*, and Lee, S.-C. (2012). The ubiquitin-like (UBX)-domain-containing protein Ubx2/Ubxd8 regulates lipid droplet homeostasis. J. Cell Sci. 125: 2930-2939.  
  • Wu, C.-H., Lee, S.-C., and Wang, C.-W.* (2011). Viral protein targeting to the cortical endoplasmic reticulum is required for cell-cell spreading in plants. J. Cell Biol.193 (3): 521-535. 
  • Lee, S.-C., Wu, C.-H., and Wang, C.-W.* (2010). Traffic of a viral movement protein complex to the highly curved tubules of the cortical endoplasmic reticulum. Traffic. 11, 912-930.  

 

Dr. Chao-Wen Wang 王昭雯
Principal Investigator
 

   

 

Robert Lin 林逸修
Research Assistant

   

 

Qmo Chen 陳聖文
Research Assistant    

 

   

Pringle Huang 黃稜潔
Research Assistant

   

Jean Chang 張景雲
Research Assistant

   

Former Lab Members

Yun-Hsin Cheng (Post-doc)
Yu-Hsuan Miao (RA)
Yi-Shun Chang (RA)
Chih-Hang Wu (RA)
Shu-Chuan Lee (Post-doc)