韋保羅

韋保羅
Verslues, Paul E.
研究員

PhD University of California-Riverside
BS, MS University of Missouri-Columbia

TEL: 

+886-2-2787-1186 (Office)
+886-2-2787-1077 (Lab)

乾旱相關的訊息傳遞和代謝

對農產業來說,植物耐旱是個重要的議題,也因為氣候變遷所造成破壞性的影響而更被重視。植物在乾旱下對水分的減少有著複雜的偵測與反應機制。由於我們仍不清楚植物如何感知缺水和啟動分子機制以適應環境並維持產率,基礎上與應用上增加經濟作物表現量的研究受到了限制。同時也有許多代謝上的改變發生在乾旱逆境下,游離的脯胺酸累積是其中最常見的。然而脯胺酸的代謝角色與其它抗旱的代謝轉變則仍不清楚。
韋保羅實驗室長期的目標是想了解仍未知的乾旱感知能力與訊息傳遞機制以及更深入地了解適應乾旱環境的代謝轉變(特別是脯胺酸代謝)及其調控。我們同時也在努力將此新機制的知識應用於改善其它植物。本實驗室專精於逆境控制測試與乾旱生理學,以及應用分子基因體學與細胞生物學技術作為研究植物逆境的方法。我們同時也與對植物逆境與代謝感興趣的定量基因體學家和生物化學家保持著合作關係。

韋保羅實驗室主要的三個互相關聯與作用的研究目標:

1.脯胺酸的逆境代謝功能及其調控。

我們試著去找出脯胺酸代謝(圖1)在乾旱逆境中是如何作出貢獻(Sharma et al., 2011; Bhaskara et al., 2015),並以脯胺酸作為乾旱感知和訊息傳遞的指標。在正向遺傳學篩選出的突變株中所發現的基因在脯胺酸去氫酶啟動子:螢光報導基因(Proline Dehydrogenase1 (PDH1) promoter:Luciferase reporter,圖2)上有著不同的表現(圖1),同時研究也發現脯胺酸合成酶P5CS1在適應乾旱環境時是如何轉變與調控植物代謝。尤其有數個特別的品系證實了更多脯胺酸所扮演的代謝角色可改變細胞中的氧化還原狀態及胞器(葉綠體及粒線體)代謝。

圖1: 脯胺酸代謝的主要途徑。P5CS1與ProDH1 (PDH1)是脯胺酸合成與分解的關鍵逆境調控酵素。

 

圖2: 突變株幼苗改變脯胺酸去氫酶啟動子:螢光報導基因之螢光酶表現圖。脯胺酸去氫酶1的表現在逆境下被抑制;然而此突變株在控制組與逆境條件下皆維持著高度脯胺酸去氫酶啟動子:螢光報導基因表現(紅色處)。此突變株與其它經篩選出類似的突變株在逆境下脯胺酸累積量皆有增加。脯胺酸去氫酶啟動子:螢光報導基因表現量減少的突變株也在此篩選中挑選出來並加以檢視。

 

2.乾旱相關的訊息傳遞機制

我們找出了AFL1(Kumar et al. 2015)以及磷酸化和細胞骨架機制等在乾旱下會促進生長的關鍵因子(圖3)。AFL1的機制以及其它經磷酸蛋白質體學與脯胺酸去氫酶啟動子:螢光報導基因篩選所找出的乾旱訊息傳遞機制也正在研究中。

圖3: 乾旱下影響植物生長的新細胞機制。

A.此圖指出AFL1在細胞膜及內膜上可能的功能,能在乾旱下擁有更好的維持生長能力。此圖出自Kumar et al., 2015。
B.早期功能未知的蛋白質磷酸酶突變株在適應乾旱時對微管構造有著更廣泛的復原能力。
 
 

3.乾旱環境適應下的自然變異表現型

乾旱導致脯胺酸累積的阿拉伯芥品系的變異中,我們找出了數個可能使得脯胺酸累積及適應乾旱的基因座(Verslues et al., 2014; Sharma et al., 2013; Kesari et al., 2012)(圖4)。目前我們正對乾旱導致的離層酸(abscisic acid; ABA)累積使用類似的方法研究中。

圖4: 不同阿拉伯芥品系的P5CS1內插子序列決定了P5CS1 後續的選擇性剪接。此變異與在不同水量下造成脯胺酸累積及環境適應的不同有所關聯。此圖來自Kesari等人,2012。

All publication list

 

Selected publication list

 

  • Kalladan R, Lasky JR, Chang TZ, Sharma S, Juenger TE, Verslues PE (2017) Natural variation identifies genes affecting drought-induced Abscisic Acid accumulation in Arabidopsis thaliana. Proceedings of the National Academy of Sciences USA  114: 11536-11541

  • Bhaskara GB, Nguyen TT, Yang T-H, Verslues PE (2017) Analysis of Phosphoproteome Remodeling After Short Term Water Stress and ABA Treatments versus Longer Term Water Stress Acclimation. Frontiers in Plant Science 8: 523

  • Wong MM, Chong GL, Verslues PE (2017) Epigenetics and RNA processing: Connections to drought, salt and ABA?  In: Methods in Molecular Biology, Plant Stress Tolerance: Methods and Protocols (Second Edition) vol 1631, Chapter 1. Sunkar, R ed, Springer

  • Verslues PE (2017) Rapid quantitation of Abscisic Acid by GC-MS/MS for studies of abiotic stress response.  In: Methods in Molecular Biology, Plant Stress Tolerance: Methods and Protocols (Second Edition), vol 1631, Chapter 21. Sunkar, R ed, Springer

  • Bhaskara GB, Wen T-N, Nguyen TT, Verslues PE (2017) Protein Phosphatase 2Cs and Microtubule-Associated Stress Protein 1 control microtubule stability, plant growth, and drought response.  Plant Cell 29: 169-191

  •  Des Marais D*, Juenger TE, Chang, TZ, Verslues PE, Lasky JR (2017) Interactive effects of water limitation and elevated temperature on the physiology, development, and fitness of diverse accessions of Brachypodium distachyon.  New Phytologist  214(1): 132-144

  •  Verslues PE (2017) Time to grow: Factors that control plant growth during mild to moderate drought stress. Plant Cell & Environment  40: 177-179  (commentary article)

  • Shinde S, Villamor JG, Lin W-D, Sharma S, Verslues PE (2016) Proline coordination with fatty acid synthesis and redox metabolism of chloroplast and mitochondria. Plant Physiology 172: 1074-1088.

  • Verslues PE (2016) ABA and cytokinins: challenge and opportunity for plant stress research. Plant Molecular Biology 91:629-640 (review)

  • Kumar MN1, Hsieh Y-F1, Verslues PE (2015) At14a-Like1 participates in membrane associated mechanisms promoting growth during drought in Arabidopsis thalianaProceedings of the National Academy of Sciences USA 112: 10545-10550 (1. Equally contributing authors)

  • Bhaskara GB, Yang T-H, Verslues PE (2015) Dynamic proline metabolism: Importance and regulation in water limited environments. Frontiers in Plant Science 6: 484. doi: 410.3389/fpls.2015.00484

  • Lovell JT*, Mullen JL, Lowry DB, Awole K, Richards JH, Sen S, Verslues PE, Juenger TE, McKay JK (2015) Exploiting differential gene expression and epistasis to discover candidate genes for drought-associated QTLs in Arabidopsis thaliana. Plant Cell 27: 969-983

  • Haswell ES, Verslues PE (2015) The ongoing search for the molecular basis of plant osmosensing.  Journal of General Physiology 145: 389–394

  • Kumar MN, Verslues PE (2015) Stress physiology functions of Arabidopsis Histidine Kinase (AHK) cytokinin receptors. Physiologia Plantarum 154: 369–380

  • Wilson ME, Basu MR, Bhaskara GB, Verslues PE, Haswell ES (2014) Plastid hypoosmotic stress activates cellular osmotic stress responses.  Plant Physiology 165: 119-128

  • Verslues PE1, Lasky JR1, Juenger TE, Liu T-W, Kumar MN (2014) Genome wide association mapping combined with reverse genetics identifies new effectors of low water potential-induced proline accumulation in Arabidopsis thalianaPlant Physiology 164: 144-159       1. Co-first authors

  • Sharma S, Shinde S, Verslues PE  (2013) Functional characterization of an ornithine cyclodeaminase-like protein of Arabidopsis thaliana.  BMC Plant Biology 13:182

  • Verslues PE, Bhaskara GB, Kesari R, Kumar MN (2013) Drought tolerance mechanisms and their molecular basis.  In: Plant Abiotic Stress-Second Edition;  Jenks, M.A. and Hasegawa, P.M. eds.; John Wilely and Sons, Inc.  (review book chapter)

  • Kumar MN, Jane W-N, Verslues PE (2013) Role of the putative osmosensor Arabidopsis Histidine Kinase 1 (AHK1) in dehydration avoidance and low water potential response.  Plant Physiology 161: 942-953   (Reccomended by Faculty of 1000)

  • Sharma S., Lin W, Villamor JG, Verslues PE (2013) Divergent low water potential response in Arabidopsis thaliana accessions Landsberg erecta and Shahdara.  Plant Cell & Environment 36: 994-1008

  • Bhaskara GB, Nguyen TT, Verslues PE (2012) Unique drought resistance functions of the Highly-ABA-Induced Clade A protein phosphatase 2Cs.  Plant Physiology 160: 379-395

  • Kesari R, Lasky JR, Villamor JG, Des Marais DL, Chen Y-JC, Liu T-W, Lin W, Juenger TE, Verslues PE (2012) Intron-mediated alternative splicing of Arabidopsis P5CS1 and its association with natural variation in proline and climate adaptation.  Proceedings of the National Academy of Sciences USA 109: 9197-9202

  • Sharma S, Villamor JG, Verslues PE (2011) Essential role of tissue specific proline synthesis and catabolism in growth and redox balance at low water potential.  Plant Physiology 157: 292-304

  • Verslues PE, Juenger TE (2011) Drought, metabolites and Arabidopsis natural variation: a promising combination for understanding adaptation to water-limited environments.  Current Opinion in Plant Biology 14: 240–245

  • Verslues PE, Sharma S (2010) Proline metabolism and its implications for plant-environment interaction.  The Arabidopsis Book 8: 140

  • Sharma S, Verslues PE (2010) Mechanisms independent of abscisic acid (ABA) or proline feedback have a predominant role in transcriptional regulation of proline metabolism during low water potential and stress recovery.  Plant Cell & Environment 33: 1838-1851

  • Verslues PE  (2010) Quantification of water stress-induced osmotic adjustment and proline accumulation for Arabidopsis thaliana molecular genetic studies.  In R. Sunkar ed.  Plant Stress Tolerance, Methods in Molecular Biology 639.  Springer Science+Business Media, LLC. (methods book chapter)

Nagaraj Kumar
拿格拉

Postdoctoral Researcher
shastishanmuga@gmail.com
 
 


Toshisangba Chuba
邱東旭

Postdoctoral Researcher
ctoshi@gmail.com
 
 
Shashi Kant Singh
夏 許

Postdoctoral Researcher
shashikanthsingh@gmail.com
 
 
Geeng Loo Chong
莊耿銣

PhD Student
gl81my@yahoo.com
 
Min May Wong
黃民妹

PhD Student
wongminmay@gmail.com
 
 
Mung Hsia Foo
胡夢霞

Research Assistant
mhfoo89@gmail.com 
 
 


Yu-Chiuan Bau
鮑昱全

Research Assistant
yuchiuan.bau@gmail.com

 
Trent Chang
張淳讓

Research Assistant
Trent1030@gate.sinica.edu.tw 
 
 
Shih-Shan Huang
黃詩珊

Lab manager
manager426@gate.sinica.edu.tw
  Dr. Paul E. Verslues