施臥虎

施臥虎
Schmidt, Wolfgang
研究員

Dr.rer.nat, Department of Biology,
University of Oldenburg, Germany (1992)

TEL: 

+886-2-2787-1162 (Office)
+886-2-2787-1038 (Lab)

Playing hard to get: The troublesome acquisition of soil-immobile nutrients

Research in my lab focuses on the elucidation of acquisition strategies for the generally soil-immobile mineral nutrients iron, phosphate and manganese. In particular, we seek to understand how plants control the uptake and distribution of these nutrients in response to changes in their availability. Such recalibration of cellular ion concentrations involves perception and communication of the nutrient status, re-programming of metabolic and developmental pathways, alterations in chromatin organization and a rearrangement of the translational machinery.

Plasticity on the move: Cell fate assignment dictated by migrating R3 MYB proteins

The fate of root epidermal cells, which differentiate into either root hair or non-hair cells, is determined by a complex interplay of edaphic genetic and systemic signals that results in a predictable but highly plastic pattern of epidermal cells that can vary in shape, size and function. Understanding the mechanisms that drive this plastic development of plants is of utmost importance against the background of a changing climate and the negative environmental aspects of intensive agriculture. In particular, we are investigating the role of the non-cell autonomous R3 MYB proteins CAPRICE and its paralogs in cell fate decisions in response to environmental cues.

 

All publication list

 

Selected publication list
  • Suen, D. F., Tsai, Y. H., Cheng, Y. T., Radjacommare, R., Ahirwar, R. N., Fu, H., & Schmidt, W. 2018. The Deubiquitinase OTU5 Regulates Root Responses to Phosphate Starvation. Plant Physiology, pp-01525

  • Yen MR, Hsu FM, Suen DF, Tsai YH, Fu H, Schmidt W, Chen PY. 2017.  OTU5 Maintains DNA Methylation Patterns and is Critical for Interpreting Phosphate Nutrition Signals. Plant Physiology, in press

  • Grillet L, Schmidt W. 2017. The multiple facets of ferric reduction by plants. Journal of Experimental Botany, in press

  • Huang J, Xue C, Wang H, Wang L, Schmidt W, Shen RF, Lan P. 2017. Genes of ACYL CARRIER PROTEIN family show different expression profiles and overexpression of ACYL CARRIER PROTEIN 5 modulates fatty acid composition and enhances salt stress tolerance in Arabidopsis. Frontiers in Plant Science, 8, 987

  • Salazar-Henao JE, Mokkapati G, Khor EHX, Chou YC, Jane WN and Schmidt W. 2017. Characterization of the spatial pattern of cell-type differentiation and morphology in the Arabidopsis root epidermis. Methods in Molecular Biology, in press.

  • Tsai, H.-H. and Schmidt, W. 2017. Mobilization of Iron by Plant-Borne Coumarins. Trends in Plant Science, invited review DOI: http://dx.doi.org/10.1016/j.tplants.2017.03.008

  • Tsai, H.-H. and Schmidt, W. 2017. One way. Or another? Iron uptake in plants. New Phytol. doi:10.1111/nph.14477

  • Jorge Rodríguez-Celma, Yi-Hsiu Tsai, Tuan-Nan Wen, Yu-Ching Wu, Catherine Curie & Wolfgang Schmidt. 2016. Systems-wide analysis of manganese deficiency-induced changes in gene activity of Arabidopsis roots. Scientific Reports 6, Article number: 35846  doi:10.1038/srep35846

  • Salazar Henao JE, Lin W.-D., Schmidt W. 2016. Discriminative gene co-expression network analysis uncovers novel modules involved in the formation of phosphate deficiency-induced root hairs in Arabidopsis. Scientific Reports, 6, 26820; doi: 10.1038/srep26820.

  • Salazar-Henao JE, Vélez-Bermúdez IC and Schmidt W. 2016. The regulation and plasticity of root hair patterning and morphogenesis. Development, 143: 1848-1858; doi: 10.1242/dev.132845

  • Jorge E. Salazar-Henao, Wolfgang Schmidt. 2016. An inventory of nutrient-responsive genes in Arabidopsis root hairs. Front. Plant Sci. 7:237. doi: 10.3389/fpls.2016.00237

  • Vélez-Bermúdez IC, Wen TN, Lan P, and Schmidt W. 2016. Isobaric tag for relative and absolute quantitation (iTRAQ)-based protein profiling in plants. Plant Proteostasis, Springer, in press.

  • Vélez-Bermúdez I-C, Salazar-Henao JE, Fornalé S, López-Vidriero I, Franco-Zorrilla J-M, Grotewold E, Gray J, Solano R, Schmidt W, Pagés M, Riera M, Caparros-Ruiz D. 2015. A MYB/ZML complex regulates wound-induced lignin genes in maize. Plant Cell 27(11):3245-59

  • Schmidt W. 2015. Root development: Pulse control. Nature Plants 1: 15148

  • Chen C-Y, Wu K, Schmidt, W. 2015. The histone deacetylase HDA19 controls root cell elongation and modulates a subset of phosphate starvation responses in Arabidopsis. Scientific Reports, Scientific Reports 5, 15708

  • Pan IC, Tsai HH, Cheng YT, Wen TN, Buckhout T, Schmidt W. 2015. Post-Transcriptional Coordination of the Arabidopsis Iron Deficiency Response is Partially Dependent on the E3 Ligases RING DOMAIN LIGASE1 (RGLG1) and RING DOMAIN LIGASE2 (RGLG2). 215. Molecular and Cellular Proteomics 14: 2733-2752

  • Lan P, Li W, Schmidt W. 2015. 'Omics' Approaches Towards Understanding Plant Phosphorus Acquisition and Use. Annual Plant Reviews 48: 65-97.

  • Chen CY, Schmidt W. 2015. The paralogous R3 MYB proteins CAPRICE, TRIPTYCHON and ENHANCER OF TRY AND CPC 1 play pleiotropic and partly non-redundant roles in the phosphate starvation response of Arabidopsis roots. Journal of Experimental Botany.doi: 10.1093/jxb/erv259.

  • Luo M, Tai R, Yu CW, Yang S, Chen C, Lin WD, Schmidt W, Wu K, 2015. Regulation of flowering time by the histone deacetylase HDA5 in Arabidopsis. The Plant Journal 82(6):925-36

  • Gollhofer J, Timofeev R, Lan P, Schmidt W, Buckhout TJ. 2014. Vacuolar-Iron-Transporter1-Like Proteins Mediate Iron Homeostasis in Arabidopsis. PLoS ONE 9(10): e110468.

  • Vélez-Bermúdez IC and Schmidt W. 2014. The conundrum of discordant protein and mRNA expression. Are plants special?. Front. Plant Sci. 5:619.

  • Schmidt W. 2014. Root systems biology. Frontiers Research Topic Ebook. ISBN: 978-2-88919-275-5

  • Pan I-C and Schmid.W. 2014. "Functional implications of K63-linked ubiquitination in the iron deficiency response of Arabidopsis roots", Frontiers in Plant Science, 4:542.

  • Grillet L, Mari S and Schmidt W. 2014. "Iron in seeds – loading pathways and subcellular localization", Frontiers in Plant Science, 4:535.

  • Wang J-Y, Lan P, Gao H-M, Zheng L, Li WF, Schmidt W. 2013. Expression changes of ribosomal proteins predict altered translation in phosphate- and iron-deficient Arabidopsis roots. BMC Genomics 14:783

  • Rodriguez-Celma J, Schmidt W. 2013. Reduction-based iron uptake revisited: on the role of secreted iron-binding compounds. Plant Signaling & Behavior 8(11)

  • Savage N, Yang JW, Chen CY, Lin K, Monk NAM, Schmidt W. 2013. Positional Signaling and Expression of ENHANCER OF TRY AND CPC1 are Tuned to Increase Root Hair Density in Response to Phosphate Deficiency in Arabidopsis thaliana. PLOS ONE 8(10)

  • Rodriguez-Celma J, Pan IC, Li W, Lan P, Buckhout TJ, Schmidt W. 2013. The transcriptional response of Arabidopsis leaves to Fe deficiency. Frontiers in Plant Nutrition 4:276

  • Lan P, Li WF, Lin W-D, Schmidt W. 2013. Mapping gene activity in Arabidopsis root hairs. Genome Biology 14(6):R67 highly accessed

  • Lan P, Li WF, Schmidt W. 2013. A digital compendium of genes mediating the reversible phosphorylation of proteins in Fe-deficient Arabidopsis roots. Frontiers in Plant Nutrition 4:173

  • Rodríguez-Celma J, Lin W-D, Fu G-M, Abadía J, López-Millán A-F, Schmidt W. 2013. Mutually exclusive alterations in secondary metabolism are critical for the uptake of insoluble iron compounds by Arabidopsis and Medicago truncatula. Plant Physiology 162(3):1473-85

  • Li WF, Lin W-D, Ray P, Lan P, Schmidt W. 2013. Genome-wide detection of condition-sensitive alternative splicing in Arabidopsis roots. Plant Physiology 162(3):1750-63

  • Buckhout, T, Schmidt W. 2013. Iron in Plants. eLS DOI: 10.1002/9780470015902.a0023713

  • Lan P, Li WF, Schmidt W. 2013. Genome-wide co-expression analysis predicts protein kinases as important regulators of phosphate deficiency-induced root hair remodeling in Arabidopsis. BMC Genomics 14:210 highly accessed

  • Sundaravelpandian K, Chandrika NNP, Schmidt W. 2013. PFT1-controlled ROS balance is critical for multiple stages of root hair development in Arabidopsis. Plant Signaling & Behavior 8(5)

  • Chandrika NN, Sundaravelpandian K, Schmidt W. 2013. A PHD in histone language: On the role of histone methylation in plant responses to phosphate deficiency. Plant Signaling & Behavior 8(6)

  • Chandrika NN, Sundaravelpandian K, Yu SM, Schmidt W. 2013.
    ALFIN-LIKE 6 is involved in root hair elongation during phosphate deficiency in Arabidopsis. New Phytologist 198(3):709-20

  • Sundaravelpandian K, Chandrika NN, Schmidt W. 2013.
    PFT1, a transcriptional Mediator complex subunit, controls root hair differentiation through reactive oxygen species (ROS) distribution in Arabidopsis. New Phytologist 197(1):151-61

  • Lan P, Li W, Schmidt W. 2012. Complementary Proteome and Transcriptome Profiling in Phosphate-Deficient Arabidopsis Roots Reveals Multiple Levels of Gene Regulation. Molecular and Cellular Proteomics 11(11):1156-66

  • Gollhofer J, Schläwicke C, Schmidt W, Buckhout TJ. 2011.Members of a small family of nodulin-like genes are regulated under iron deficiency in roots of Arabidopsis thaliana. Plant Physiology and Biochemistry 49(5): 557-564

  • Liao YY, Buckhout TJ, Schmidt W. 2011. Phosphate deficiency-induced cell wall remodeling – linking gene networks with polysaccharide meshworks. Plant Signaling & Behavior 6(5)

  • Lin W, Liao YY, Yang JW, Pan CY, Buckhout T, Schmidt W. 2011. Coexpression-based Clustering of Arabidopsis Root Genes Predicts Functional Modules in Early Phosphate Deficiency Signaling. Plant Physiology 155: 1383-1402

  • Lan P, Schmidt W. 2011. The enigma of eIF5A in the iron deficiency response of Arabidopsis. Plant Signaling & Behavior 6(4)

  • Lan P,Li W, Wen TN, Shiau JY, Wu YC, Lin W, Schmidt W.2011. iTRAQ protein profile analysis of Arabidopsis roots reveals new aspects critical for Fe homeostasis. Plant Physiology 155: 821-834

  • Schmidt W, Buckhout T. 2011. A hitchhiker's guide to the Arabidopsis ferrome. Plant Physiology and Biochemistry 49(5): 462-470

  • Yang JW, Lin W, Schmidt W. 2010. Transcriptional Profiling of the Arabidopsis Iron Deficiency Response Reveals Conserved Transition Metal Homeostasis Networks. Plant Physiology 152: 2130-2141

 

   

Pei-Ru Liao 廖珮如
Lab Manager

tjb@gate.sinica.edu.tw

   

Isabel Cristina Vélez Bermüdez 貝翊婷
Postdoc

isabelvelezbermudez@gmail.com

   
Louis Grillet 盧毅
Postdoc

louis_grillet@hotmail.fr

   
Jorge Enrique Salazar Henao 何瑞恩
Postdoc

jesalazarhenao@gmail.com

   
Lisa Hsieh 謝恩蓉
Postdoc

persimmon04@gate.sinica.edu.tw

   

Chandan Kumar Gautam 高千譚
PhD Student

ckgautam@gate.sinica.edu.tw

   
Girish Mokkapati 紀律希
PhD Student

girishmokkapati@gmail.com

   
Yi-Hsiu Tsai 蔡宜秀
PhD student

yisho1108@gmail.com

   
Feng Mei Lien 連鳳美
Assistant

avilta0218@yahoo.com.tw

   
Cassie Cheng 鄭雅丹
Research Assistant

cassie_giyabin@hotmail.com

   
Merrisa Tsai 蔡慧萱
PhD student

merrisa_tsai@hotmail.com