鄭萬興

鄭萬興
Cheng, Wan-Hsing
副研究員

Ph.D. Plant Molecular and Cellular Biology Program University of Florida
M.S. Horticulture National Taiwan University
B.S. Horticulture National Chung-Hsing University

TEL: 

+886-2-2787-1177 (Office)
+886-2-2787-1065 (Lab)

研究主題:植物荷爾蒙離層酸、生物逆境、和它們的反應

植物荷爾蒙離層酸(abscisic acid, ABA)擔任重要角色在調控種子發育、休眠、氣孔關閉、和逆境抗性(圖A)。我們先前研究顯示ABA2, 又稱為葡萄糖不敏感基因(glucoseinsensitive1,gin1), 轉譯一個短鏈去氫/ 還原酶(SDR1), 在ABA生合成路徑中, 此酶催化xanthoxin成離層醛(abscisic aldehyde)。ABA2在長期逆境下,為一晚表現基因,具有微調控ABA生合成功能。阿拉伯芥過度表現ABA2之轉殖株,會造成ABA累積、種子發芽晚、和耐鹽類等特性。有趣地,ABA-調控百合異形葉型(heterophylly) 的產生與差異性表現ABA生合成基因NCED3 有關。此外,離層酸vs. 乙烯(ethylene) 或離層酸vs. 吉貝酸(GA) 具有相互拮抗功能而影響種子發芽,幼苗生長和葉片發育;兩者相互調控對方生合成、代謝、或信息傳遞,進而增強彼此的相互拮抗。

在非生物逆境的研究方面,我們用正向遺傳的方法,篩選出10 個鹽類過度敏感株(salt hypersensitive mutant, sah)(圖B), 其中,sah1和sah9具有很強的鹽類過度敏感、植株小、短根、和不正常花粉型態等生長缺陷。這兩個蛋白質主要表現於核仁(nucleolus)。sah1和sah9除了對鹽類過度敏感外,在葉脈、葉肉細胞、和對植物生長激素(auxin)的反應,也具有缺陷。它們具有組織核醣體(ribosomal assembly)之功能;而對鹽類過度敏感的機制,部分出自於改變microtubule穩定性和鹽類相關基因表現有關。

雖然離層酸生合成和它的receptor基因大部分已被選殖和定性,但是ABA和逆境信息傳遞間之交互反應,仍然被了解不多。為了更進一步了解ABA和逆境信息傳遞間之交互反應,我們利用遺傳學方式,篩選出幾個aba2 suppressor (abs) 突變株(圖C)。這些abs對葡萄糖或鹽類之感應度與正常株相同;不過,若生長在土壤上,這些abs性狀則與aba2和正常株不同。相信此研究對於ABA和養份或鹽類逆境之交互反應機制,會有重大之新發現。

All publication list

 

Selected publication list

 

  • Chiang MH, Shen HL, and Cheng WH.* (2015) Genetic analyses of the interaction between abscisic acid and gibberellins in the control of leaf development in Arabidopsis thaliana. Plant Science 236:260-271.
     
  • Chen YH, Shen HL, Hsu PJ, Hwang SG, and Cheng WH.* (2014) N-acetylglucosamine-1-P uridylytransferase 1 and 2 are required for gametogenesis and embryo development in Arabidopsis thaliana. Plant Cell Physiol. 55:1977-1993.
     
  • Hsu YF, Chen YC, Hsiao YC, Wang BJ, Lin SY, Cheng WH, Jauh GY, Harada JJ, and Wang CS. (2014) AtRH57, a DEAD RNA helicase, is involved in feedback inhibition of glucose-mediated abscisic acid accumulation during seedling development and additively affects pre-ribosomal RNA processing with high glucose. Plant J. 77:119-135
     
  • Hwang SG, Lin NC, Hsiao YY, Kuo CH, Chang PF, Deng WL, Chiang MH, Shen HL, Chen CY, and Cheng WH.* (2012) The Arabidopsis short-chain dehydrogenase/reductase 3, an ABSCISIC ACID DEFFICIENT 2 homolog, is involved in plant defense responses but not in ABA biosynthesis. Plant Physiol Biochem. 51:63-73.
     
  • Chen HC, Hwang SG, Chen SM, Shii CT, and Cheng WH.* (2011) ABA-mediated heterophylly is regulated by differential expression of 9-cis-expoxycarotenoid dioxygenase 3 in lilies. Plant Cell Physiol. 52:1806-1821.
     
  • Hwang SG, Chen HC, Huang WY, Chu YC, Shii CT, and Cheng WH.* (2010) Ectopic expression of rice OsNCED3 in Arabidopsis increases ABA level and alters leaf morphology. Plant Sci. 178: 12-22.
     
  • Cheng WH*, Chiang MH, Hwang SG, and Lin PC. (2009). Antagonism between abscisic acid and ethylene in Arabidopsis acts in parallel with the reciprocal regulation of their metabolism and signaling pathways. Plant Mol. Biol. 71: 61-80.
     
  • Lin PC, Hwang SG, Endo A, Okamoto M, Koshiba T, and Cheng WH.* (2007) Ectopic expression of ABA2/GIN1 in Arabidopsis promotes seed dormancy and stress tolerance. Plant Physiol. 143: 745-758.

博士後研究 Postdoctoral research Associate
江明豪 Ming-Hau Chiang

研究助理 Research Assistant
沈惠齡 Hwei-Ling Shen

博士班研究生 Ph. D. Student
陳雅惠 Ya-Huei Chen
黃楷超 Kai-Chau Huang
林威至 Wei-Chih Lin