陳忠謀

陳忠謀
Chen, Chung-Mong
副研究員

美國北卡羅來那大學教堂山分校生化及生物物理系博士(1987)

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尋找影響阿拉伯芥品種Col-type和Ws-type telomere3R長度的基因

阿拉伯芥品種Columbia (Col)和Wassilewskija (Ws)在第三條染色體右端(3R)的端粒相鄰序列(telomere-associated sequence, TAS)彼此不同,端粒長度在前者不同個體間的分佈為2-4 kb,後者為4-9 kb (Wang et al., 2010; 圖一)。為了區別這兩種telomere3R,我們把連著Col3RTAS的端粒稱為Col-type telomere3R,連著Ws3RTAS的端粒稱為Ws-type telomere3R。用適當DNA引子及單一端粒長度分析技術(single telomere length analysis, STELA)可以從混合的Col+Ws基因組DNA清楚鑑定Col-type和Ws-type telomere3R的長度(圖一)。為探討Col及Ws品種控制telomere3R長度的基因,我們用STELA追蹤單一Col和Ws植株本身及它們雜交產生的F1及F2的telomere3R長度(圖二)。每株F1具有Col-type和Ws-type telomere3R各一條,雖然各F1植株的基因組合不盡相同,但F1絕大多數Col-type及Ws-type telomere3R的長度分別和其親代類似,顯示Col-type及Ws-type telomere3R的長度控制機制互不相同;F1植株中約有2.8%到3.5% Ws-type telomere3R呈現Col-type telomere3R的長度,10% Col-type telomere3R呈現Ws-type telomere3R的長度,這種現象在親代自花授粉後代中並沒有見到,顯示某些Col-Ws基因組合影響Col-type或Ws-type telomere3R的長度。4株同時具有Col-type和Ws-type telomere3R的F2植株中,它們的Col-type telomere3R都維持親代Col-type telomere3R的長度,但有兩株的Ws-type telomere3R呈現Col-type telomere3R的長度,顯示Col-type telomere3R(或Col3RTAS)和Ws-type telomere3R(或Ws3RTAS)可能分別帶有影響本身端粒長度的不同信息,因此在同一植株或遺傳背景下前者和後者長度有不同變化。由F2經多代自花授粉已產生200株重組純系(recombinant inbreed lines),每株都只有Col-type或Ws-type telomere3R。分析它們的telomere3R長度和68個標示DNA分佈的關係將找出影響Col-type或Ws-type telomere3R長度的基因。

Fig 1. (A) Sequence associated with telomere 3R in the ecotype Col (Col-type 3RTAS) differs from that in the ecotype Ws (Ws-type 3RTAS).  (B) The length of Col-type and Ws-type telomere 3R in a sample containing both ecotypes of genomic DNA can be measured unambiguously by single telomere length analysis (STELA). (C) The length of Col-type and Ws-type telomere 3R in an Arabidopsis population maintained by self-pollination ranges, respectively, from 2 to 4 kb and 4 to 9 kb.

 

Fig 2. Tracing by STELA the length distribution of both Col-type and Ws-type telomere 3R among individual Arabidopsis plants including (A)a single plant of both Col and Ws ecotypes and the progenies generated by self-fertilization of each ecotype plant, (B)several F1 plants obtained by a cross-fertilization between both ecotype plants and (C)some F2 plants derived from one of the F1 plant shown on (B).

 

All publication list

 

Selected publication list

 

  • Wang CT, Ho CH, Hseu MJ, and Chen CM.* (2010). The subtelomeric region of the Arabidopsis thaliana chromosome IIIR contains potential genes and duplicated fragments from other chromosomes. Plant Mol. Biol. 74: 155-166.
  • Chen, C. M., Wang, C. T., and Ho, C. H. 2001. A plant gene encoding a Myb-like protein that binds telomeric GGTTTAG repeats in vitro. J. Biol. Chem. 276: 16511-16519.
     
  • Lin, T. Y., Kao, Y. Y., Lin, S., Lin, R. F., Chen, C. M., Huang, C. H., Wang, C. K., Lin, Y. Z., and Chen, C. C. 2001. Construction of a genetic map for Nicotiana plumbaginifolia and comparison of it with the synteny groups of N. sylvestris. Theor. Appl. Genet. 103: 905-911.
     
  • Hsu, F. C.,Wang, C. J., Chen, C. M., Hu, H. Y., and Chen, C. C. 2003. Molecular characterization of a family of tandemly repeated DNA sequences, TR-1, in heterochromatic knobs of maize and its relatives. Genetics 164: 1087-1097.
     
  • Chen, C. M., Wang, C. T., Kao, Y. H., Chang, G. D., Ho, C. H., Lee, F. M., and Hseu, M. J. 2005. Functional reududancy of the duplex telomeric DNA-binding proteins in Arabidopsis. Bot. Bull. Acad. Sinica 46:315-324.
     
  • Sue, S. C., Hsiao, H. H., Chung, C. P. B., Cheng, Y. H., Hsueh, K. L., Chen, C. M., Ho, C. H., and Huang, T. H. 2006. Solution structure of the DNA binding domain of the telomeric repeat-binding protein of Arabidopsis thaliana: a new fold with an additional C-terminal helix. J. Mol. Biol. 356: 72-85.
     
  • P.V. Palani, M. Chiu, W. Chen, C.C. Wang, C.C. Lin, C.C. Hsu, C.P. Cheng, Chen. C.M. , Y.H. Hsu, N.S. Lin.. Subcellular localization and expression of bamboo mosaic virus satellite RNA-encoded protein. JOURNAL OF GENERAL VIROLOGY. 90, 507-518., 2009.
     
  • Wang, C. T., Ho, C. H., Hseu, M. J., and Chen, C. M. 2010. The subtelomeric region of the Arabidopsis thaliana chromosome IIIR contains potential genes and duplicated fragments from other chromosomes. Plant Mol. Biol. 74: 155-166.
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Research Assistant 研究助理
Chi-Ting Wang 王琪婷