符宏勇

符宏勇
Fu, Hong-yong
副研究員

美國德州農工大學博士 (1995)

TEL: 

+886-2-2787-1183 (Office)
+886-2-2787-1074 (Lab)

研究主題: 泛素/26S蛋白酶體系統主要組成因子之功能與機制探討

泛素與26S蛋白酶體系統係所有真核生物生長發育極重要之調控機制。研究室目前之研究方向包括:主要泛素受體之生化與功能分析、OTU去泛素酵素家族成員之功能分析、及RUF RING-型泛素黏合酵素家族之功能分析。闡明系統組成因子之功能與作用機制可增進對植物學各層面之瞭解,並可提供作物改良之基因操控策略。

主要泛素受體之生化與功能分析

泛素化受質辨識係由演化上十分保守之多重泛素受體所組成之不同辨識途徑所執行,我們比較不同物種泛素受體之生化特性發現主要泛素受體之辨識途徑具有明顯歧異性,顯示物種間其功能與機制之差異。為在單一物種內探討不同泛素化受質辨識途徑之功能與作用機制,我們進一步利用交互作用及遺傳方法分析阿拉伯芥主要泛素受體,包括蛋白酶體次單元 RPN10、RPN13及含UBL與UBA結構區之RAD23、DSK2、DDI1及NUB1等。雖然受質交互作用分析顯示RPN10、RAD23及 DSK2應是阿拉伯芥主要受體,剔除突變株系表型分析顯示除了剔除株系 rpn10-2之外,其餘株系均具野生表現型。出乎預期地,受質辨識結構區定位突變之RPN10可以完全互補rpn10-2之所有嚴重表型缺失。結果顯示阿拉伯芥泛素化受質辨識是由多重功能重疊之泛素受體所仲介以確保該步驟執行。相對地,未變性電泳分析顯示 rpn10-2剔除株系中雙帽蛋白酶體(RP2-CP)量明顯減少,顯示結構缺失可能是造成其剔除表型之主要因素。

嶄新去泛素酵素OTU家族成員之功能分析

研究室發現一含OTU結構區之去泛素酵素家族。親緣關係、生化特性及突變表型分析顯示OTU成員具不同功能。其中OTU5參與主要開花抑制因子FLCMAF4MAF5之轉錄活化而抑制開花。證據顯示OTU5直接參與這些開花抑制基因之組蛋白修飾,雖突變表型與參與組蛋白H2A.Z置放之SWR1複體次單元突變幾乎相同,生化與分子遺傳證據顯示OTU5存在另一蛋白複體上。

The elusiveness of in vivo roles of major ubiquitin receptors is highlighted by complementation experiments showing that the conjugate binding activity of the Arabidopsis proteasomal ubiquitin receptor RPN10 is not required to complement its null mutant phenotypes

 

All publication list

 

Selected publication list

 

  • Radjacommare R, Usharani R, Kuo CH, Lin WD, Jauh GY, Fu H*. (2015) Arabidopsis ovarian tumor-related deubiquitinase OTU5 activates flowering repressors FLC, MAF4, and MAF5 by histone modification to suppress flowering (The Plant Cell, encourage resubmit/under revision).

  • Fu H*, Goring D, Genschik P. (2015). eBook: Reversible Ubiquitylation in Plant Biology. Front. Plant Sci.

  • Radjacommare R, Usharani R, Kuo C, Fu H*. (2014) Distinct phylogenetic relationships and biochemical properties of Arabidopsis ovarian tumor-related deubiquitinases support their functional differentiation. Front. Plant Sci. 5:84.

  • Shin LJ, Lo JC, Chen GH, Callis J, Fu H, Yeh KC*. (2013) IRT1 degradation factor 1, a ring E3 ubiquitin ligase, regulates the degradation of iron-regulated transporter 1 in Arabidopsis. The Plant Cell 25: 3039-3051.

  • Lin YL, Fu H*. (2012) In vivo relevance of substrate recognition function of major Arabidopsis ubiquitin receptors. Plant Signal. Behav. 7:722-727. (most popular download, Jul-2012)

  • Lin YL, Sung SC, Tsai HL, Yu TT, Radjacommare R, Usharani R, Fatimababy AS, Lin HY, Wang YY, Fu H*. (2011) The defective proteasome but not substrate recognition function is responsible for the null phenotypes of the Arabidopsis proteasome subunit RPN10. The Plant Cell 23: 2754-2773.

  • Fu H*, Lin YL, Fatimababy AS. (2010) Feature review: Proteasomal recognition of ubiquitylated substrates. Trends Pl. Sci.15: 375-386.

  • Fatimababy AS, Lin YL, Usharani R, Radjacommare R, Wang HT, Tsai HL, Lee Y, Fu H*. (2010) Cross-species divergence of major recognition pathways of ubiquitylated substrates for ubiquitin/26S proteasome-mediated proteolysis. FEBS J.277: 796-816.

  • Farmer LM, Book AJ, Lee KH, Lin YL, Fu, H, Vierstra RD*. (2010) The RAD23 family provides an essential connection between the 26S proteasome and ubiquitylated proteins in Arabidopsis. The Plant Cell 22: 124-142.

  • Chang LC, Guo CL, Lin YS, Fu H, Wang CS, Jauh GY*. (2009) Pollen-specific SKP1-like proteins are components of functional SCF complexes and essential for lily pollen tube elongation. Plant Cell Physiol. 50: 1558-1572.

博士後研究 Postdoctoral research Associate
林詩芸 Shih Yun Lin
沈毓星 Yu-Hsing Shen

研究助理 Research Assistant
林碧蓮 Jessica Krisanti Gusman
焦沙茹 Saruny George

博士班研究生 Ph. D. Student
瑪莉塔 Marita Anggarani
拉姆 Ram Nivas Ahirwar