林納生

林納生
Lin, Na-Sheng
特聘研究員

美國內不拉斯加大學博士 (1984)

TEL: 

+886-2-2787-1128 (Office)
+886-2-2787-1022 (Lab)

I病毒與宿主間之分子交互作用

病毒為宿主細胞的絕對寄生物,會挾持受感染之宿主細胞的生理活動迫使其致力於病毒的複製或移動以完成其生活史。然而,植物亦會激發植物賀爾蒙傳導路徑來活化抗病毒基因表現,並利用基因靜默機制來抵禦病毒的感染。此外,當兩種以上不同病毒感染同一植物,常會引起協力作用而加速病毒的複製,是故病毒與植物宿主間之攻防戰存在複雜且緊密之交互關係。我們以感染竹類之竹嵌紋病毒(BaMV)和蘭花最常見之兩種病毒─東亞蘭嵌紋病毒(CymMV)及齒舌蘭輪斑病毒(ORSV)─為研究材料。結合次世代定序分析、遺傳、生化、分生及細胞生物學等方法探討BaMV如何挾持宿主因子或內生性微小RNA,參與其複製及移動;也探討植物離層酸如何活化基因靜默重要因子ARGONAUTE蛋白而抑制病毒之累積。我們以蘭花全方位轉錄體解析CymMV和ORSV之病毒協力作用與蝴蝶蘭的免疫抗性之分子機制,並開發具病毒抗性之轉殖植物。

II. 病毒、衛星核酸與宿主之交互作用

病毒雖為宿主寄生物,然有些病毒會攜帶次病毒分子,例如衛星核酸(satellite RNA)。它與協助病毒雖無序列同源性,但演化出相似之二級RNA結構,以仰賴病毒進行複製及包被。有趣的是,部分干擾型衛星核酸會抑制協助病毒之複製及病徵表現。過去,我們篩選出干擾型之竹嵌紋病毒衛星核酸(satBaMV),並定位出其抑制BaMV複製之主要決定因子及其分子機制。長期目標上我們想了解衛星核酸是否利用與病毒相同或不同之宿主因子以完成其生活史。近年來我們發現satBaMV藉由fibrillarin核仁蛋白之幫助可在植物體中移動,但fibrillarin並不為病毒移動所需。因此,我們的研究將可提供病毒、衛星核酸與宿主三者交互作用分子機制更深入的探討。

Fig. (A) BaMV atomic model showing N- and C-terminal extensions that allow flexibility of the virions,
            and left-handed helical with ~8.8 subunits per turn.
      (B) Synergistic mixed infection of CymMV and ORSV enhanced symptom formation in Phalaenopsis.
      (C) Our research interests focus on trilateral interaction among virus, satellite RNA, and host.

All publication list

 

Selected publication list

 

  • publication list (2011~2017)

  • Chiang-Hsieh, Y. F., Pai, H., Tseng K. C., Lee S. C., Zheng H. Q., Lin N. S.,* and Chang W. C.* 2017. MicroRPM: An effective model for novel microRNA prediction based only on small RNA sequencing data without reference genome. Bioinformatics. 2017 Nov 9. doi: 10.1093/bioinformatics/btx725. [Epub ahead of print]
     
  • Alazem M, Lin N. S.* 2017. Antiviral Roles of Abscisic Acid in Plants. Front Plant Sci. 8:1760.
     
  • Huang, Y. W., Hu, C.C., Tsai, C.H., Lin, N. S., Hsu, Y.H. 2017. Chloroplast Hsp70 isoform is required for age-dependent tissue preference of Bamboo mosaic virus in mature Nicotiana benthamiana Leaves. Mol Plant Microbe Interact. 30(8): 631-645.
     
  • Huang, Y. W., Hu, C. C., Hsu, Y. H. and Lin, N. S.* 2017. Replication of plant viral satellites. InViroids and Plant Viral Satellites (edited by Drs. Hadidi, Flores, Randles, and Palukaitis), Academic Press (Invited).
     
  • Liou, M. R., Hu, C. C., Lin, N. S. and Hsu, Y. H.* 2017. Development and application of satellite-based vectors. InViroids and Plant Viral Satellites (edited by Drs. Hadidi, Flores, Randles, and Palukaitis), Academic Press (Invited).
     
  • Chang, K. C., Chang, L. T., Huang, Y. W., Lai, Y. C., Lee, C. W., Liao, J. T., Lin, N. S., Hsu, Y. H., Hu, C.C. 2017. Transmission of Bamboo mosaic virus in bamboos mediated by insects in the Order Diptera. Front Microbiol.8:870.
     
  • Wang, I. N.*, Yeh, W. B, Lin, N. S. 2017. Phylogeography and Coevolution of Bamboo Mosaic Virus and Its Associated Satellite RNA. Front Microbiol. 8: 886.
     
  • Chen, T. H., Hu, C. C., Liao, T. L., Lee, Y. L., Huang, Y. W., Lin, N. S., Y. L., Hsu, Y. H.* 2017. Production of Japanese encephalitis virus antigens in plants using bamboo mosaic virus-based vector. Front Microbiol. 8: 788.
     
  • Lin, K. Y.* and Lin, N. S. 2017. Interfering satellite RNAs of Bamboo mosaic virus. Front Microbiol. 8: 787.
     
  • Alazem M, He, M. H., Moffett, P., Lin, N. S.* 2017. Abscisic acid induces resistance against Bamboo mosaic virus through Argonaute 2 and 3. Plant Physiology 174: 339–355.
     
  • Alazem M., and Lin, N. S.* 2017.  Large Satellite RNAs. InViroids and Plant Viral Satellites (edited by Drs. Hadidi, Flores, Randles, and Palukaitis), Academic Press (Invited).
     
  • Ho, T. L., Lee, H. C., Chou, Y. L., Tseng, Y. H., Huang ,W. C., Wung, C. H., Lin, N. S., Hsu, Y. H., Chang, B. Y.* 2017. The cysteine residues at the C-terminal tail of Bamboo mosaic virus triple gene block protein 2 are critical for efficient plasmodesmata localization of protein 1 in the same block. Virology 501:47-53.
     
  • Chang, C. H., Hsu, F. C., Lee, S. C., Lo, Y. S., Wang, J. D., Shaw, J., Taliansky, M., Chang, B. Y., Hsu, Y. H., Lin, N. S.* 2016. The nucleolar fibrillarin protein is required for helper virus-independent long-distance trafficking of a subviral satellite RNA in plants. Plant Cell 28:2586-2602.
     
  • Cheng, N., Lo Y. S., Ansari, M. l., Ho, K. C., Jeng, S. T., Lin, N. S.*, Dai, H.* 2016. Correlation between mtDNA complexity and mtDNA replication mode in developing cotyledon mitochondria during mung bean seed germination. New Phytologist doi: 10.1111/nph.14158.
     
  • DiMaio F., Chen, C. C., Yu X., Frenz B., Hsu, Y. H.*, Lin, N. S.*, Egelman E. H.* 2015.The molecular basis for flexibility in the flexible filamentous plant viruses. Nat Struct Mol Biol. 22:642-644.
     
  • Alazem, M., and Lin, N. S.* 2015. Roles of plant hormones in the regulation of host-virus interactions. Mol Plant Pathol. 16:529-540.
     
  • Wang, I. N., Hu,C. C., Lee, C. W., Yen, S. M., Yeh, W. B., Hsu, Y. H., and Lin, N. S.* 2014. Genetic Diversity and Evolution of Satellite RNAs Associated with the Bamboo Mosaic Virus. PLoS ONE 9(9):e108015.
     
  • Liou, M. R., Huang, Y. W., Hu, C. C., Lin, N. S.*, and Hsu, Y. Y.* 2014. A dual gene-silencing vector system for monocot and dicot plants. Plant Biotech. J. 12(3):330-43.
     
  • Alazem, M., Lin, K. Y., and Lin, N. S.* 2014. The abscisic acid pathway has multifaceted effects on the accumulation of Bamboo mosaic virus. Mol Plant-Microbe Interact. 27(2):177-89.
     
  • Lin, K. Y., Hsu, Y. Y., Chen, H. C. and Lin, N. S.* 2013. Transgenic resistance to Bamboo mosaic virus by expressing interfering satellite RNA. Mol. Plant Path. 14: 693-707.
     
  • Chen, H. C., Kong, L. R., Yeh, T. Y., Cheng, C. P., Hsu, Y. H. and Lin, N. S.* 2012. The conserved 5' apical hairpin stem loops of bamboo mosaic virus and its satellite RNA contribute to replication competence. Nucleic Acids Res. 40(10): 4641-4652.
     
  • Lin, N. S. 2012. Development of Bamboo mosaic virus as a plant expression vector for functional study of genes involved in bamboo flowering. In: Tandon, V. and Barik, S. K. Editors, Bamboo flowering and rodent control. pp. 119-123. Astral International Pvt. Ltd. New Delhi.
     
  • Chen, T. Y. and Lin, N. S.* 2011. Transgenic protection against virus infection in orchids. Orchid Biotechnology II. Chapter 18. Edited by H. H. Chen. World Scientific Publishing Co. Pte. Ltd. (Invited)
     
  • Vetten, H. J., Scholthof, K. B. G., Lin, N. S., Briddon, R. W., Ghabrial, S. A., and  Palukaitis, P. 2011. Satellites and Other Virus-dependent Nucleic Acids. In: King, A. M. G., Adams, M. J., Carstens, E. B. and Lefkowitz, E. J. Editors, Virus Taxonomy. Oxford: Elsevier, pp. 1209-1220. ISBN: 978-0-12-384684-6. Elsevier Inc. Elsevier. (Invited).
     
  • Vijayapalani, P., Chen, C. F., Liou, M. R., Hsu, Y. H. and Lin, N. S.* 2011. Phosphorylation of Bamboo mosaic virus satellite RNA (satBaMV)-encoded protein P20 downregulates the formation of the satBaMV-P20 ribonucleoprotein complex. Nucleic Acids Res. 40(2): 638-649.

林冠妤 Kuan-yu Lin

博士後研究

   

李淑娟 Shu-chuan Lee

博士後研究

   

王俊達 Jun-da Wang

博士後研究

   

成寧 Ning Cheng

博士後研究

   

陳弈仲 Yi-chun Chen

博士後研究

   

廖麗施 Alice Liao

研究助理

   

陳婷玉 Ting-yu Chen

研究助理

   

白暄 Hsuan Pai

研究助理

   

何孟勳 Meng-hsun He

研究助理

   

王智緯 Chih-wei Wang

研究助理

   

陳相伃 Xiang-yu Chen

研究助理

   

錢衡 Heng Qian

大專生

   

陳揚升 Yang-sheng Chen

大專生