鄒稚華

鄒稚華
Tsou, Chih-Hua
副研究員

美國紐約大學博士 (1990)

TEL: 

+886-2-2787-1187 (Office)
+886-2-2787-1078 (Lab)

本研究室近年來的工作以花藥的發育為重心。花藥是植物產生及散播花粉的器官,花藥發育的報告相當豐富,但受限於一般顯微鏡學製備過程,有些題目極少被報導;如花藥內小孢子在脫離胼胝體層時的分佈、花藥腔的液體何時乾燥、花藥開裂時水分快速流動的過程等都未能即時且真實的呈現。另外,花粉依賴營養層提供營養,但養份在二者之間傳輸的途徑有不同的說法,藉著藥腔液體流動是廣為接受的說法,但也有養分經由微管狀的結構運輸的說法。我們利用本所的冷凍掃描電鏡及穿透式電鏡搭配高壓冷凍固定的方法,針對禾本科作物,主要是玉米進行研究。

我們觀察到禾本科花藥腔中小孢子/花粉一直圍成一圈緊貼著營養層直到花藥開裂早期;禾本科的花藥空腔在減數分裂早期開始形成、擴大並充滿液體,到小孢子大液泡期則開始失水變乾。花藥腔乾燥後,緊貼在花藥壁上的一圈花粉仍持續發育7-9天,並正常地儲藏滿滿的澱粉粒而達成熟。在禾本科中,我們沒有發現任何管狀結構存在於營養層與花粉之間。由於在許多種的花藥腔中會見到一些花粉散在花藥腔中間,與周圍的正常花粉接觸,卻未與營養層有直接接觸。通常至花藥開裂時,這些中央花粉仍維持活性但與正常花粉比較,中央花粉體積較小,不含或僅含少量澱粉粒。中央花粉一旦曝露在大氣中,會因含水量高而即刻萎縮,置於培養基上亦無法萌發。由結果推測:在藥壁內面及花粉表面有一層薄薄的界面液體,水分及蔗糖等養分可以經由這層界面液體由花藥壁流入花粉,而花粉壁上密佈的細小圓柱型徑向分布的通道可為花粉攝入物質的途徑。蔗糖被緊貼著營養層的正常花粉大量吸收,因此中央花粉無法製造大量的澱粉。在玉米花藥開裂時首先藥隔細胞瓦解造成大裂縫 隨之營養層膜也被斷開 因此相鄰的二個孢子囊會相通 接著藥壁開始撐大至半圓狀 最後藥壁細胞自頂端開始脫水 造成花藥孔打開並擴大花藥開裂終至完成。

All publication list

 

Selected publication list

 

  • Tsou CH, Tseng CM, Fu YL, Yen HJ, You TR, Walden DB and Cheng PC. Insights into maize anther development and dehiscence as revealed by cryo-SEM. (Submitted to American Journal of Botany)
     
  • Su MH, Wang JC, Hsieh CF and Tsou CH*. (2012) A taxonomic study of Camellia brevistyla and C. tenuiflora (Theaceae) based on phenetic analyses. Botanical Studies 53: 275-283.
     
  • Vijayan K, Chung MC and Tsou CH*. (2012) Dispersion of rDNA loci and its implications on intragenomic variability and phylogenetic studies in Camellia. Scientia Horticulturae. 137: 59-68.
     
  • Vijayan K and Tsou CH*. (2010) DNA barcoding in plants: Taxonomy in a new perspective. Current Science. 99: 1530-1541.
     
  • Vijayan K, Zhang WJ and Tsou CH*. (2009) Molecular taxonomy of Camellia (Theaceae) inferred from nrITS sequences. American Journal of Botany. 96: 1348-1360.
     
  • Vijayan K and Tsou CH*. (2008) Phylogenetic implications of nrITS on Camellia: The need of high quality DNA and PCR amplification with Pfu-DNA polymerase. Botanical Studies. 49: 177-188.
     
  • Tsou CH and Mori SA. (2007). Floral organogenesis and floral evolution of the Lecythidoideae (Lecythidaceae). American Journal of Botany. 94: 716-736.
     
  • Wu CC, Hsu ZF and Tsou CH*. (2007). Phylogeography of Eurya (Ternstroemiaceae) from Taiwan as inferred from ITS sequences. Botanical Studies. 48:97-166.
     
  • Mori SA, Tsou CH, Wu CC, Cronholm B and Anderberg AA. (2007). Evolution of Lecythidaceae with an emphasis on the circumscription of Neotropical genera. Information from combined ndhF and trnL-F sequence data. American Journal of Botany. 94: 289-301.
     
  • Tsou CH* and Fu YL. (2007). Octad pollen formation in Cymbopetalum (Annonaceae): the binding mechanism. Plant Systematics and Evolution. 263:13-23.

Lab members

Research Assistants
Yu-Lan FU
Hsiao-Jung YEN

Postdoctoral Fellow
Chi-Chih WU