涂世隆

涂世隆
Tu, Shih-Long
副研究員

國防醫學院生命科學研究所博士 (2001)

TEL: 

+886-2-2787-1167 (Office)
+886-2-2787-1045 (Lab)

植物生長與分化容易受到環境變化影響, 為了能因應環境的變化,植物已知可藉由訊息 接收及傳遞,在不同層面來調控基因表現以控 制生理及形態,目前相關研究相當多,但在訊 息RNA剪接層面調控的機制了解卻很少。

變異型RNA剪接 (alternative splicing) 是 真核生物中常見的機制,可以利用同樣的訊 息RNA 前驅物 (pre-mRNA) ,在不同的剪接位 進行剪接後,形成多條的訊息RNA,此機制 大幅增加了轉錄體 (transcriptome) 及蛋白質體 (proteome) 的複雜度及多樣性。變異型RNA剪 接可被剪接調控蛋白 (splicing regulator) 及調控 序列 (regulatory cis element) 所影響,這幾年植 物的轉錄體的相關研究雖已發現存在大量的變 異型訊息RNA,然而對變異型RNA剪接的調控 機制所知不多,其是否對植物抗逆境反應有幫 助亦是未知。

我們近幾年利用系統生物學的研究方法, 探討環境因子對植物體內變異型RNA剪接的影 響。除了證實光線的確調控特定基因群的變異 型RNA剪接,也發現光受器 (photoreceptor) 參 與調控機制。目前我們希望利用功能性分析、 生物化學及系統生物學的研究方法,探討光受 器控制訊息RNA剪接的分子機制及其生理意 義。希望這些研究可以幫助了解光受器調控訊 息RNA剪接模式,以及此機制在植物的演化過 程,最終利用此調控機制控制農作物的生長及 分化。

光敏素調控mRNA剪接的分子機制

All publication list

 

Selected publication list

 

  • Wu HP, Su YS, Chen HC, Chen YR, Wu CC, Lin WD, Tu SL*. (2014) Genome-wide analysis of light-regulated alternative splicing mediated by photoreceptors in Physcomitrella patens. Genome Biol. 15:R10.

  • Chang, C.-Y., Lin, W.-D., and Tu, S.-L. (2014). Genome-wide analysis of heat-sensitive alternative splicing in Physcomitrella patens. Plant Physiol. 165, 826-840.

  • Wu, H.-P., Su, Y.-s., Chen, H.-C., Chen, Y.-R., Wu, C.-C., Lin, W.-D., and Tu, S.-L. (2014). Genome-wide analysis of light-regulated alternative splicing mediated by photoreceptors in Physcomitrella patens. Genome Biol. 15, R10.

  • Chen, Y.-R., Su, Y.-s., and Tu, S.-L. (2012). Distinct phytochrome actions in non-vascular plants revealed by targeted inactivation of phytobilin biosynthesis. Proc. Natl. Acad. Sci. USA. 109, 8310-8315.
     
  • Chiu, F.-Y., Chen, Y.-R., and Tu, S.-L.(2010). Electrostatic interaction of phytochromobilin synthase and ferredoxin for biosynthesis of phytochrome chromophore. J. Biol. Chem. 285, 5056-5065.
     
  • Tu, S. L., Chen, H. C., and Ku, L. W. (2008). Mechanistic studies of the phytochromobilin synthase HY2 from Arabidopsis. J. Biol. Chem. 283, 27555-27564.
     
  • Tu, S. L., Rockwell, N. C., Fisher, A. J., and Lagarias, J. C. (2007). Insight into the radical mechanism of phycocyanobilin:ferredoxin oxidoreductase (PcyA) revealed by X-ray crystallography and biochemical measurements. Biochemistry. 46, 1484-1494.
     
  • Tu, S. L. , Sughrue, W., Britt, R. D., and Lagarias, J. C. (2006). A conserved histidine-aspartate pair is required for exo-vinyl reduction of biliverdin by a cyanobacterial phycocyanobilin:ferredoxin oxidoreductase. J Biol. Chem. 281, 3127-3136.
     
  • Tu, S. L., and Lagarias, J. C. (2005). The phytochromes. In Handbook of Photosensory Receptors, W. R. Briggs, and J. A. Spudich, eds. (Weinheim, Germany, Wiley-VCH).
     
  • Tu, S. L., Chen, L. J., Smith, M. D., Su, Y. S., Schnell, D. J., and Li, H. M. (2004a). Import pathways of chloroplast interior proteins and the outer-membrane protein OEP14 converge at Toc75. The Plant Cell. 16, 2078-2088.
     
  • Tu, S. L., Gunn, A., Toney, M. D., Britt, R. D., and Lagarias, J. C. (2004b). Biliverdin reduction by cyanobacterial phycocyanobilin:ferredoxin oxidoreductase (PcyA) proceeds via linear tetrapyrrole radical intermediates. J Am. Chem. Soc. 126, 8682-8693.
     
  • Chou, M. L., Fitzpatrick, L. M., Tu, S. L., Budziszewski, G., Potter-Lewis, S., Akita, M., Levin, J. Z., Keegstra, K., and Li, H. M. (2003). Tic40, a membrane-anchored co-chaperone homolog in the chloroplast protein translocon. EMBO J. 22, 2970-2980.
     
  • Sun, Y. J., Forouhar, F., H.M., L., Tu, S. L., Yeh, Y. H., Kao, S., Shr, H. L., Chou, C. C., Chen, C., and Hsiao, C. D. (2002). Crystal structure of pea Toc34, a novel GTPase of the chloroplast protein translocon. Nat. Struct. Biol. 9, 95-100.
     
  • Tu, S. L., and Li, H. M. (2000). Insertion of OEP14 into the outer envelope membrane is mediated by proteinaceous components of chloroplasts. The Plant Cell. 12, 1951-1960.

 

     
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博士後研究員

 

     
陳薌雯
研究助理

 

     
謝欣宇
研究助理

 

     
張平
博士班研究生

 

     
林渤昀
博士班研究生

 

     
施玨如
博士班研究生

 

     
王健彰
博士班研究生